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山东大学学报 (医学版) ›› 2021, Vol. 59 ›› Issue (5): 52-59.doi: 10.6040/j.issn.1671-7554.0.2021.0220

• 医学病毒的基础与临床研究进展专题 • 上一篇    下一篇

中国南方三省H10亚型禽流感病毒基因特征分析

曾晓旭,张恒,焦铭,陈涛,李希妍,黄维娟,杨静,王大燕*()   

  1. 中国疾病预防控制中心病毒病预防控制所 国家流感中心, 北京 102206
  • 收稿日期:2020-03-01 出版日期:2021-05-10 发布日期:2021-06-01
  • 通讯作者: 王大燕 E-mail:dayanwang@cnic.org.cn
  • 基金资助:
    国家科技重大专项(2018ZX10711-001);国家自然科学基金(81961128002)

Genetic characteristics of H10 subtype avian influenza virus in three southern provinces of China

Xiaoxu ZENG,Heng ZHANG,Ming JIAO,Tao CHEN,Xiyan LI,Weijuan HUANG,Jing YANG,Dayan WANG*()   

  1. Chinese National Influenza Center, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
  • Received:2020-03-01 Online:2021-05-10 Published:2021-06-01
  • Contact: Dayan WANG E-mail:dayanwang@cnic.org.cn

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摘要：

目的: 分析2014~2019年我国南方三省H10亚型禽流感病毒的基因特征。方法: 采集2014~2019年江西、湖南和广西3个省份活禽市场环境样本共计30231份，采用实时荧光定量PCR对上述环境样本进行甲型禽流感病毒筛查，阳性样本进一步做病毒分离，若病毒分离结果为阳性，则对其进行基因深度测序。利用CLC、CD-HIT、MAFFT v7.037和MEGA v6.06软件分析流感病毒序列。结果: 三省禽流感强化监测共分离到11株H10亚型禽流感病毒。通过全基因组测序和系统进化分析发现，除3个N8基因位于北美分支，其他基因均位于欧亚分支。JX24472与人H10N8亚型禽流感病毒HA基因片段亲源关系较近。江西分离的5株H10N3亚型禽流感病毒内部基因片段较为分散，内部基因片段来源呈多样性。病毒蛋白位点分析结果显示部分潜在的与受体结合位点、小鼠致病性以及在哺乳动物中的传播性有关的氨基酸位点发生突变。结论: 本研究分离的11株H10亚型禽流感病毒与人H10N8亚型禽流感病毒基因处于不同分支(JX24472除外)，但是氨基酸位点分析提示这些病毒具有潜在使人或者禽感染并致病的可能性，应持续对其加强监测，为潜在的流感大流行做准备。

关键词: 禽流感病毒, H10N3, H10N8, 进化分析, 活禽市场, 监测

Abstract:

Objective: To explore the genetic characteristics of avian influenza virus (AIVs) between 2014 and 2019 in three southern provinces of China. Methods: A total of 30 231 environment samples isolated from Jiangxi, Hunan and Guangxi provinces from 2014 to 2019 were tested for flu A with real-time RT-PCR. If the testing results were positive, the samples were propagated in SPF chicken embryonated eggs. All positive AIVs isolations were sequenced. CLC, CD-HIT, MAFFT v7.037 and MEGA v6.06 software were used to analyze the influenza sequences. Results: Altogether 11 H10 subtype AIVs were isolated. Whole genome sequencing and phylogenetic analysis revealed that all genes were classified into Eurasian lineage, except 3 N8 genes in the North American lineage. JX24472 had close relationship with human infection H10N8 AIVs in its HA segment. The internal genome of 5 H10N3 subtype AIVs isolated from Jiangxi Province suggested their genome were diverse and complex. Molecular characteristic analysis of H10 subtype AIVs revealed that several mutations in key amino acid sites were associated with receptor binding sites, pathogenicity in mice and transmissibility in mammals. Conclusion: Phylogenetic analyses of the 11 H10 subtype AIVs isolated in this research suggested that they were in different clade with human infected H10N8 AIVs, except JX24472. Whereas the amino acid characterization analysis suggested that these H10 subtype viruses had the potential to infect human or poultry to make them sick. Therefore, it is necessary to strengthen the continuous surveillance of H10 subtype AIVs and prepare for the next potential pandemic.

Key words: Avian influenza virus, H10N3, H10N8, Phylogenetic analysis, Live poultry market, Surveillance

中图分类号: 

  • R373.1

表1

H10亚型禽流感病毒样本信息"

毒株名称 缩写 亚型 采样日期 采样场所 样本种类
A/chicken/Jiangxi/24472/2014 JX24472 H7/H10/N9 2014-04-29 活禽零售市场 鸡泄殖腔
A/environment/Jiangxi/07531/2015 JX07531 H10N3 2015-11-19 鸭养殖场 鸭粪便
A/environment/Jiangxi/07505/2015 JX07505 H10N3 2015-11-19 鸭养殖场 鸭笼具
A/environment /Jiangxi/07518/2015 JX07518 H10N3 2015-11-19 鸭养殖场 鸭粪便
A/environment /Jiangxi/07527/2015 JX07527 H10N3 2015-11-19 鸭养殖场 鸭粪便
A/environment/ Jiangxi/12590/2019 JX12590 H10N3 2019-06-18 批发市场 鸭粪便
A/environment/Guangxi/13425/2018 GX13425 H10N3 2018-12-24 活禽零售市场 脱毛机
A/environment/Guangxi/25019/2019 GX25019 H10N3 2019-01-02 活禽零售市场 鸭粪便
A/environment/Hunan/12257/2019 HN12257 H10N8 2019-08-19 活禽零售市场 禽咽拭子
A/environment/Hunan/12259/2019 HN12259 H10N8 2019-08-19 活禽零售市场 禽咽拭子
A/environment/Hunan/12263/2019 HN12263 H10N8 2019-08-19 活禽零售市场 鸭笼具

图1

HA基因进化树"

图2

NA基因进化树 A：N3基因进化树; B：N8基因进化树。"

图3

H10Nx内部基因进化树 A：PB2基因进化树; B：PB1基因进化树; C：PA基因进化树; D：NP基因进化树; E：M基因进化树; F：NS基因进化树。"

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