间充质干细胞对肠道病毒71型的易感性及miRNA的差异表达

doi:10.6040/j.issn.1671-7554.0.2016.1372

摘要/Abstract

摘要： 目的分析miRNA在肠道病毒71型(EV71)感染间充质干细胞后的表达差异,探讨miRNA在EV71感染中的作用机制。方法 EV71体外感染脐带间充质干细胞(MSC)建立EV71感染模型,采用基因芯片技术分析EV71感染MSC前后miRNA的差异表达,通过生物信息学分析其相关信号通路。结果 EV71感染MSC前后有84个miRNA发生了异常变化,主要涉及MAPK、Wnt等信号通路。结论 MSC细胞对EV71有易感性;EV71通过miRNA调控MAPK、Wnt等信号通路参与EV71感染。

关键词: 手足口病, MAPK信号通路, miRNAs, 肠道病毒7l型

Abstract: Objective To explore the functions of microRNA(miRNA)in Enterovirus 71(EV71)infection in human mesenchymal stem cells(MSCs). Mehods The EV71 infection model was established by using umbilical cord derived MSC in vitro, then the expression level of miRNAs was characterized via microarray platform and the related signaling pathways were revealed through bioinformatics analysis. Results Totally 84 differentially expressed miRNAs were found, mainly involved in the signaling pathway of the MAPK, Wnt and etc. Conclusion MSCs have a clear susceptibility to EV71, revealing that the EV71 infection can be regulated by using MAPK and Wnt signaling pathways via miRNAs.

Key words: Enterovirus 71, miRNAs, Hand, foot and mouth disease, MAPK signaling pathway

中图分类号:

R574

邓轲,刘毅,盖中涛. 间充质干细胞对肠道病毒71型的易感性及miRNA的差异表达[J]. 山东大学学报（医学版）, 2017, 55(3): 25-31.

DENG Ke, LIU Yi, GAI Zhongtao. Susceptibility of mesenchymal stem cells to enterovirus 71 and differential expression of miRNA[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(3): 25-31.

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