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山东大学学报（医学版） ›› 2016, Vol. 54 ›› Issue (9): 37-40.doi: 10.6040/j.issn.1671-7554.0.2016.096

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肿瘤微环境中TGF β-1对子宫内膜间质细胞活化的影响

路真真,颜磊,张辉,张晓晖,赵兴波   

  1. 山东大学附属省立医院妇产科, 山东 济南 250021
  • 收稿日期:2016-01-26 出版日期:2016-09-10 发布日期:2016-09-10
  • 通讯作者: 赵兴波. E-mail:zxb0626@126.com E-mail:zxb0626@126.com
  • 基金资助:
    国家自然科学基金(81272858)

Effects of TGF β-1 on the activation of endometrial stromal cells in tumor microenvironment

LU Zhenzhen, YAN Lei, ZHANG Hui, ZHANG Xiaohui, ZHAO Xingbo   

  1. Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China
  • Received:2016-01-26 Online:2016-09-10 Published:2016-09-10

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摘要： 目的 探讨子宫内膜癌肿瘤微环境及转化生长因子β-1(TGF β-1)对正常间质细胞(NF)活化为肿瘤相关成纤维细胞(CAF)的影响。 方法 (1)体外培养人子宫内膜癌细胞系Ishikawa细胞,制备条件培养基(CM),原代培养NF,形成共培养体系,Western blotting与QT-PCR方法检测其α-平滑肌肌动蛋白(α-SMA)的表达。(2)分组为:A:NF,B:NF+TGF β-1,C:NF+CM,D:NF+CM+TGF β-RⅠ/Ⅱ激酶抑制剂。 结果 B和C组α-SMA蛋白表达较A组高(P=0.01、P<0.001), B和C组α-SMA的mRNA表达较A组高,两组比较差异有统计学意义(P<0.001、P=0.01);B和C组α-SMA蛋白表达较D组高(P<0.001、P=0.01),B和C组α-SMA的mRNA表达较D组高,两组比较差异有统计学意义(P<0.001、P=0.01)。与C组相比,B组α-SMA蛋白表达较C组高(P<0.001)。B组与C组比较α-SMA mRNA表达差异无统计学意义(P=0.40)。 结论 子宫内膜癌肿瘤微环境可促进NF-CAF的转化,TGFβ-1在该过程中发挥重要作用。

关键词: 肿瘤相关成纤维细胞, 肿瘤微环境, α-平滑肌肌动蛋白, 子宫内膜癌

Abstract: Objective To investigate the effects of endometrial carcinoma microenvironment and transforming growth factor β-1(TGF β-1)on the activation of normal endometrial fibroblasts(NF)to carcinoma-associated fibroblasts(CAF). Methods Endometrial carcinoma cell line(Ishikawa cells)was cultured in vitro. Conditioned medium(CM)was prepared. Primarily cultured NF was co-cultured. The cells were divided into four groups and treated accordingly: NF group(group A), NF+TGF β-1(group B), NF+CM(group C), and NF+CM+TGF β-R I/II kinase inhibitors(group D). The expression of α-smooth muscle actin(α-SMA)was detected with QT-PCR and Western blotting. Results The α-SMA protein and mRNA expressions in group B and C were higher than those in group A(P=0.01, P<0.001; P<0.001, P=0.01). The α-SMA protein and mRNA expressions in group B and C were higher than those in group D(P<0.001, P=0.01; P<0.001, P=0.01). Compared with group C, group B had higher α-SMA protein expression(P<0.001), but the α-SMA mRNA expression had no statistical difference(P=0.40). Conclusion Endometrial carcinoma microenvironment can promote the transformation of NF-CAF, and TGFβ-1 plays an important role in the process.

Key words: Carcinoma-associated fibroblasts, Endometrial carcinoma, α-smooth muscle actin, Endometrial carcinoma microenvironment

中图分类号: 

  • R711.74
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