白细胞介素33在脑血管病患者血清中的表达及其作用机制

doi:10.6040/j.issn.1671-7554.0.2014.711

摘要/Abstract

摘要： 目的探讨脑血管疾病患者血清中白细胞介素33(IL-33)水平与颈动脉粥样硬化斑块稳定性及脑梗死的关系及其作用机制.方法采用ELISA方法检测脑梗死组(n=63)、短暂性脑缺血发作组(n=30)及正常对照组(n=15)血清中IL-33的表达水平;采用Western blotting、荧光定量PCR(qRT-PCR)和ELISA法检测体外培养的人外周血单个核细胞(PBMC)和U937细胞在接受外源性IL-33刺激后,MMP-9和Cox-2的表达量变化.结果脑梗死组IL-33的表达水平低于对照组和短暂性脑缺血发作组(P均 <0.01);脑梗死组中不稳定斑块、稳定斑块和无斑块患者血清中IL-33的表达水平逐渐降低(P <0.01);脑梗死组中,与无糖尿病和高血脂症的患者相比,合并糖尿病和高脂血症的患者血清中IL-33水平降低(P均 <0.01);外源性IL-33刺激可下调外周血单核细胞和U937细胞中MMP-9和Cox-2的表达和分泌量.结论 IL-33可被作为预测脑梗死及不稳定性粥样硬化斑块的潜在的血清标志物和治疗靶点.

关键词: 外科手术, 白细胞介素33, 动脉粥样硬化, 基质金属蛋白酶9, 血清, 亲情护理服务, 心理护理

Abstract: Objective To explore the prognostic value of serum interleukin-33(IL-33) level in cerebral infarction with carotid atherosclerotic plaque and the pathological functions of IL-33. Methods ELLSA was applied to detect serum IL-33 levels in the patients with cerebral infarction(n=63)and transient ischemic attack(n=30), as well as normal controls(n=15). Western blotting,qRT-PCR and ELISA were used to detect the expression levels of MMP9 and Cox-2 in PBMC and U937 cells after IL-33 stimulation. Results The serum level of IL-33 was lower in patients with cerebral infarction, compared with those in transient ischemic attack and normal controls(all P<0.01). IL-33 expression levels decreased in turn in cerebral infarction patients with unstable plaque, stable plaque and without plaque(P <0.01). IL-33 expression level was also lower in the serum of cerebral infarction patients concurrent with diabetes and hyperlipidaemia(all P <0.01). Furthermore, MMP9 and Cox-2 expression levels in PBMC and U937 could be down-regulated after treatment with IL-33. Conclusion IL-33 can be regarded as a potential serum marker or treatment target of cerebral infarction and unstable atherosclerotic plaques.

Key words: Atherosclerosis, Surgery, Matrix metalloproteinase 9, Interleukin-33, Serum, Psychological nursing, Kindred-like nursing

中图分类号:

R743

于磊, 李振. 白细胞介素33在脑血管病患者血清中的表达及其作用机制[J]. 山东大学学报（医学版）, 2015, 53(4): 75-79.

YU Lei, LI Zhen. Expression of interleukin-33 and its function in the serum of patients with cerebrovascular disease[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(4): 75-79.

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