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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (4): 6-11.doi: 10.6040/j.issn.1671-7554.0.2014.826

• 基础医学 • 上一篇    下一篇

SN-38缓释系统的制作及其抗胶质瘤作用的体外实验研究

朱晓东, 夏同良, 姚庆宇, 李昊元, 王本琳, 倪石磊, 王建刚, 李新钢, 苏万东   

  1. 山东大学齐鲁医院神经外科 山东大学脑科学研究所, 山东 济南 250012
  • 收稿日期:2014-11-17 修回日期:2015-03-09 出版日期:2015-04-10 发布日期:2015-04-10
  • 通讯作者: 苏万东.E-mail:suwandong@sina.com E-mail:suwandong@sina.com
  • 基金资助:
    山东大学自主创新基金(自然科学)(2012TS157);中国博士后科学基金面上项目(20100471526);国家自然科学基金面上项目(81472353)

SN-38-loaded electrospun composite nanofiber scaffolds for the treatment of human glioma cells in vitro

ZHU Xiaodong, XIA Tongliang, YAO Qingyu, LI Haoyuan, WANG Benlin, NI Shilei, WANG Jiangang, LI Xingang, SU Wandong   

  1. Department of Neurosurgery, Qilu Hospital of Shandong University; Institute for Brain Science, Shandong University, Jinan 250012, Shandong, China
  • Received:2014-11-17 Revised:2015-03-09 Online:2015-04-10 Published:2015-04-10

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摘要： 目的 以聚己内酯/外消旋聚乳酸(PCL/PDLLA)为载体材料,制作安全有效的载有7-乙基-10-羟基喜树碱(SN-38)的缓释系统,评价对U-251胶质瘤细胞的抗肿瘤效果.方法 通过电纺丝方法制作SN-38 -PCL/PDLLA纺丝膜,采用差示扫描热分析法 (DSC)及傅立叶转换红外线光谱(FTIR)分析SN-38在载药聚合物纤维中的状态;采用接触角测量评价载药纤维的亲疏水性;在体外观察不同材料纤维的药物释放速率及对U-251胶质瘤细胞的抑制作用.结果 载药纺丝膜形态均一,在体外实验中均表现出一定的持续抑制胶质瘤细胞的能力,由于载药量的不同,表现出不同的释放时间.其中2%载药的静电纺丝膜表现出稳定、持续的抗肿瘤活性,突释不明显.结论 应用PCL/PDLLA制备搭载SN-38的缓释系统是可行的,适当提高载药量可以增加药物释放时间.

关键词: 外消旋聚乳酸, 胶质瘤, 聚己内酯, 电纺丝, 7-乙基-10-羟基喜树碱

Abstract: Objective To establisht an effectively controlled release system which was based on 7-ethyl-10-hydroxyl camptothecin (SN-38)-loaded electrospun composite nanofiber with poly (ε-caprolactone) (PCL)/poly (D, L-lactide) (PDLLA) and to evaluate its antitumor activity against U-251 glioma cells in vitro. Methods SN-38-loaded PCL/PDLLA fibers were manufactured by electrospinning. The physical status of SN-38 within the polymeric fibers was investigated by different scanning calorimetry (DSC) and fourier transform infrared (FT-IR). The static wettability of the membrane was measured by contact angle analyzer. The release speeds of two samples were determined, and their cytotoxicity against U-251 glioma cells was assessed in vitro. Results Uniform and smooth SN-38-loaded PCL/ PDLLA fibers were obtained. Cell toxicity test results showed that the SN-38-loaded PCL/PDLLA fibers could continuously inhibit the U-251 glioma cells. Due to different drug-loading rates, different membranes showed various release time. The antitumor activity of the 2 wt.% SN-38-loaded PCL/PDLLA nanofiber meshes were controlled and sustained. Conclusions Using SN-38-loaded composite nanofiber of PCL/PDLLA as a sustained delivery system is feasible. While the drug-loading rate is augmented, the release time of drug within the fibers will be increased accordingly.

Key words: Poly (ε-caprolactone), Electrospinning, Glioma, Poly (D,L-lactide), 7-ethyl-10-hydroxyl camptothecin

中图分类号: 

  • R739.41
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