乌苯美司诱导肾癌细胞死亡的作用及机制

doi:10.6040/j.issn.1671-7554.0.2015.415

山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (9): 58-64.doi: 10.6040/j.issn.1671-7554.0.2015.415

乌苯美司诱导肾癌细胞死亡的作用及机制

黄智龙^1,2, 刘帅¹, 王建伟¹, 韩立平³, 王晓庆¹, 李新¹, 毕东滨¹, 解放⁴, 牛志宏¹

1. 山东大学附属省立医院泌尿外科, 山东济南 250021;
2. 兰陵县人民医院泌尿科, 山东兰陵 277700;
3. 山东省警官总医院, 山东济南 250002;
4. 威海市立医院泌尿外科, 山东威海 264200

收稿日期:2015-04-05 出版日期:2015-09-10 发布日期:2015-09-10
通讯作者: 牛志宏。E-mail:nzh1789@163.com E-mail:nzh1789@163.com
基金资助:
山东省自然科学基金(ZR2014HM111,ZR2014HP015)

Role and possible mechanism of autophagy in ubenimex-induced cell death of renal cell carcinoma

HUANG Zhilong^1,2, LIU Shuai¹, WANG Jianwei¹, HAN Liping³, WANG Xiaoqing¹, LI Xin¹, BI Dongbin¹, XIE Fang⁴, NIU Zhihong¹

1. Department of Urology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
2. Department of Urology Lanling County People's Hospital, Lanling 277700, Shandong, China;
3. Shandong Police Hospital, Jinan 250002, Shandong, China;
4. Department of Urology, Weihai Municipal Hospital, Weihai 264200, Shandong, China

Received:2015-04-05 Online:2015-09-10 Published:2015-09-10
Contact: 牛志宏。E-mail:nzh1789@163.com E-mail:nzh1789@163.com

摘要/Abstract

摘要： 目的观察乌苯美司对肾癌细胞增殖及死亡的影响并探讨其可能机制。方法利用WST-8细胞增殖实验及细胞生长曲线检测乌苯美司对肾癌细胞增殖能力的影响;LDH释放实验检测乌苯美司对肾癌细胞死亡的影响;酶活性测定法及Western blotting研究乌苯美司诱导肾癌细胞APN活性及表达;Western blotting及免疫组化染色观察乌苯美司的作用靶点氨肽酶N(APN)/CD13在肾癌组织及正常组织中的表达。结果乌苯美司不能降低肾癌细胞中APN的表达但是可以抑制其活性;乌苯美司可抑制肾癌细胞增殖及活力,诱导细胞自噬性死亡。结论乌苯美司可抑制肾癌细胞增殖,并可通过诱导自噬引发肾癌细胞死亡。

关键词: 氨肽酶N, 细胞死亡, 肾癌, 乌苯美司, 自噬

Abstract: Objective To determine the effect of ubenimex on the proliferation of renal cell carcinoma cells and the possible mechanism. Methods WST-8 cell proliferation assay and cell growth curve were used to detect the proliferation of renal cell carcinoma cells. LDH cytotoxicity assay was used to detect the cell death of the renal cell carcinoma. Alanine-p-nitroanilido was used to detect the activity and expression of APN induced by ubenimex in renal cell carcinoma cell lines. Western blotting and immunohistochemical staining were used to detect the expression of APN/CD13 in tissues of normal kidney and renal cell carcinoma. Results Ubenimex could not reduce the express of APN in renal cell carcinoma but inhibit its activity. Ubenimex could inhibit the proliferation and activity of renal cell carcinoma cells, and induce autophagic cell death. Conclusion Ubenimex inhibits the proliferation of renal cell carcinoma cells and promotes levels of cell death in renal cell carcinoma cells via an autophagic mechanism.

Key words: Renal cell carcinoma, Autophagy, Aminopeptidase N, Ubenimex, Cell death

中图分类号:

R692

黄智龙, 刘帅, 王建伟, 韩立平, 王晓庆, 李新, 毕东滨, 解放, 牛志宏. 乌苯美司诱导肾癌细胞死亡的作用及机制[J]. 山东大学学报（医学版）, 2015, 53(9): 58-64.

HUANG Zhilong, LIU Shuai, WANG Jianwei, HAN Liping, WANG Xiaoqing, LI Xin, BI Dongbin, XIE Fang, NIU Zhihong. Role and possible mechanism of autophagy in ubenimex-induced cell death of renal cell carcinoma[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(9): 58-64.

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乌苯美司诱导肾癌细胞死亡的作用及机制

Role and possible mechanism of autophagy in ubenimex-induced cell death of renal cell carcinoma

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