关键词: T24细胞, 明胶酶A, 膀胱肿瘤, 肝素裂合酶, RNA干扰

Abstract: To explore the changes of proliferation and MMP-2 expression in T24 cells after siRNA downregulating heparanase (Hpa) gene on bladder cancer cell line-T24. MethodsCells were divided into six groups: the normal control group, the blank control group, the negative control group, the Hpa-siRNAⅠ group, the Hpa-siRNAⅡ group, and the Hpa-siRNAⅢ group. Chemically synthesized small interfering RNA (siRNA) silencing Hpa gene was transfected into the human bladder cancer cell line-T24. Then proliferation of T24 cells was determined by MTT assay, expression of Hpa mRNA was determined by reverse transcription polymerase chain reaction (RT-PCR), and expression of MMP-2 was determined by immunocytochemistry. ResultsMTT showed that the growth of T24 cells was decreased by (28.55±3.66)%, (27.20±3.25)% and (37.29±4.82)% in the three experimental groups. RT-PCR analyses also showed that the Hpa mRNA level was significantly decreased in some experimental groups compared with the control groups. Also immunocytochemical staining showed that the MMP-2 (Gelatinase A) expressions were much lower in the experimental group than those in the control groups(P＜0.05). Conclusions RNA interference (RNAi) could significantly decrease T24 cell proliferation and the Hpa gene mRNA level, and inhibits the MMP-2 expression in the bladder cancer cell line-T24.

Key words: RNA interference, Heparinase, Gelatinase A, Bladder neoplasms, T24 cells