NOS/NO和HO/CO系统及药物干预在实验性兔动脉粥样硬化进程中的作用

山东大学学报(医学版)

NOS/NO和HO/CO系统及药物干预在实验性兔动脉粥样硬化进程中的作用

刘同涛¹，于清霞²，田庆印¹，孙春丽¹，李伯勤³

1. 山东大学齐鲁医院心内科，济南 250012； 2. 烟台毓璜顶医院ICU, 山东烟台 264000；3. 山东大学医学院解剖与组织胚胎学研究所，济南 250012

收稿日期:2007-11-27 修回日期:1900-01-01 出版日期:2008-06-16 发布日期:2008-06-16
通讯作者: 刘同涛

Medication intervention on heme oxygenase/carbon monoxide and nitric oxide synthase/nitric oxide systems in the atherosclerosis model of rabbits

LIU Tong-tao¹，　YU Qing-xia²,　TIAN Qing-yin¹,　SUN Chun-li¹,　LI Bo-qin³

1. Department of Cardiology, Qilu Hospital of Shangdong University, Jinan 250012, China；2. Department of ICU, Yantai Yuhuangding Hospital, Yantai 264000, Shandong China; 3. Institute of Histology and Embryology

Received:2007-11-27 Revised:1900-01-01 Online:2008-06-16 Published:2008-06-16
Contact: LIU Tong-tao

摘要/Abstract

摘要： 目的探讨动脉粥样硬化进程中一氧化氮合酶/一氧化氮（NOS/NO）和血红素加氧酶/一氧化碳（HO/CO）系统的变化、相互关系以及辛伐他汀对该系统的影响。方法16只家兔予以高胆固醇饮食喂养8周，停用高胆固醇饮食后，随机分为辛伐他汀组（n=8）和模型组（n=8）。辛伐他汀组喂饲辛伐他汀进行药物干预，继续给予普通饲料喂养8周；同时设正常对照组（n=8），给予普通饲料喂养16周。然后取静脉血和主动脉组织，分别用沉淀漂浮酶联法、Chalmers A H、硝酸还原酶法测定各实验组血中TC、TG、LDL、HDL、CO、 NO含量，免疫组织化学方法测定主动脉组织中诱导型一氧化氮合酶（iNOS）和血红素加氧酶1（HO1）的表达水平；并比较两组间各项参数的差异。结果8周末与对照组比较，模型组血脂水平明显升高,血清NO含量明显降低，血浆CO水平明显升高（P均＜0.01）。16周末与模型组比较，辛伐他汀组血浆TC、TG、LDL明显下降（P＜0.01）,HDL和血清NO含量明显升高（P＜0.01），血浆CO水平明显降低（P＜0.01），HO-1及iNOS表达明显减少（P＜0.01）。结论动脉粥样硬化进程中，HO/CO和NOS/NO系统显示出互补及代偿性调节作用，辛伐他汀可以通过下调HO/CO和NOS/NO系统而延缓动脉粥样硬化进程。

关键词: 一氧化氮, 一氧化碳, 一氧化氮合酶, 血红素加氧酶, 血红素加氧酶, 辛伐他汀, 免疫组织化学, 动脉硬化

Abstract: To investigate changes and intercorrelation of heme oxygenase-1(HO-1)/carbon monoxide(CO) and nitric oxide synthase (NOS)/nitric oxide(NO) and the effect of simvastatin on them in atherosclerotic progress. Methods16 rabbits had a 1% cholesterol diet for eight weeks and after eight weeks were fed with normal diets for eight weeks and then were divided into two groups: the simvastatin group and the model group, 8 rats fed with normal diets served as the control group. Levels of serum lipids, serum NO and plasma CO were determined at the beginning, the 8th week and the 16th week, and also the expressions of HO-1 and NOS in the thoracia aortic tissues were determined by the immunohistochemistry technique. ResultsAt the end of the 8th week, levels of serum lipids and plasma CO were increased in the simvastatin group (P＜0.01) and the level of serum NO and expressions of HO-1 and inducible nitric oxide synthase (iNOS) were decreased compared with the control group (P＜0.01). At the end of the 16th week, levels of serum lipids and plasma CO and expressions of HO-1 and iNOS were significantly decreased (P＜0.01), with the model group. Conclusions In atherosclerotic progress, HO1/CO and NOS/NO systems have a reciprocal relationship, and simvastatin may delay the atherosclerotic progress by decreasing the two systems.

Key words: Atherosclerosis, Heme oxygenase, Carbon monoxide, Nitric oxide synthase, Nitric oxide, Simvastatin, Immunohistochemistry

中图分类号:

R541.4

刘同涛,于清霞,田庆印,孙春丽,李伯勤 . NOS/NO和HO/CO系统及药物干预在实验性兔动脉粥样硬化进程中的作用[J]. 山东大学学报(医学版), 2008, 46(6): 566-570.

LIU Tong-tao,YU Qing-xia,TIAN Qing-yin,SUN Chun-li,LI Bo-qin. Medication intervention on heme oxygenase/carbon monoxide and nitric oxide synthase/nitric oxide systems in the atherosclerosis model of rabbits[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(6): 566-570.

参考文献

相关文章 15

[1]	黄方康瑞吴春林. VEGFC、NF-κBp65及Survivin在鼻咽癌中的表达及临床意义[J]. 山东大学学报(医学版), 2209, 47(6): 83-.
[2]	郝跃伟刘雪平赵婷婷郑敏王一兵. 环氧化酶2基因多态性与动脉粥样硬化缺血性脑卒中的相关性[J]. 山东大学学报(医学版), 2209, 47(6): 95-98.
[3]	高秀蕊臧恒昌. 复方烟酸辛伐他汀双层片体外释放度的研究[J]. 山东大学学报(医学版), 2209, 47(6): 109-113.
[4]	曹洛菲,王珊珊,王金荣,姜荷云,苗瑜,马光增. 哮喘发作期FeNO升高儿童肺功能舒张试验的特点分析[J]. 山东大学学报 (医学版), 2025, 63(6): 38-44.
[5]	张如岗,殷悦,李岱云,段梦琦,刘桂莲,王瑛. 支原体感染对成人肺功能及呼出气一氧化氮的影响[J]. 山东大学学报 (医学版), 2025, 63(3): 71-75.
[6]	王莉莎,王上增,史栋梁,张仲博,任博文,王云飞,郭中华,周晓宁. 乌头汤对膝骨关节炎大鼠模型的治疗作用[J]. 山东大学学报 (医学版), 2024, 62(5): 64-71.
[7]	魏闫若雪,李梓绮,刘春铖,刘晓晗,赵然,刘玉昆. 结直肠癌中SP1的瘤内异质性表达及其临床意义[J]. 山东大学学报 (医学版), 2024, 62(5): 89-94.
[8]	刘春铖,刘晓晗,魏闫若雪,李梓绮,刘玉昆,赵然. 结直肠癌中含溴结构域蛋白9的亚细胞定位模式及其临床意义[J]. 山东大学学报 (医学版), 2024, 62(4): 24-30.
[9]	吴霖,石晨曦,童星. 肺良性转移性平滑肌瘤病7例并文献复习[J]. 山东大学学报 (医学版), 2024, 62(4): 78-84.
[10]	樊荣,李彬彬,马晓丽,汪运山,郏雁飞. 胃癌中DEC2、HIF-2α的表达及临床意义[J]. 山东大学学报 (医学版), 2023, 61(7): 12-18.
[11]	李宁,王翠兰,张玉荣. 帕金森病患者脑动脉多普勒测定指数及血清表皮生长因子与动脉硬化和认知障碍的关联分析[J]. 山东大学学报 (医学版), 2021, 59(3): 61-66.
[12]	何鹏娟,颜磊,范明君,刘祥斌,赵兴波. 辛伐他汀对TGF-β诱导的卵巢癌上皮间质转化的影响[J]. 山东大学学报 (医学版), 2018, 56(5): 46-51.
[13]	聂思月,王震,孙靖宇,刘杰,李娟,魏小娟,路春晓,吕兴龑,王淑云,孙玉萍. ANGPTL5在人胃癌组织中的表达及临床意义[J]. 山东大学学报（医学版）, 2017, 55(8): 61-65.
[14]	李景媛,宋玲,林松,宋晖. 不同浓度一氧化碳释放分子-3对大鼠骨髓间充质干细胞成骨分化的影响[J]. 山东大学学报（医学版）, 2017, 55(7): 31-37.
[15]	宋昱,毛洋,刘庆梅,乔磊,帖媛媛,鲁璐,张蕾. 通心络对兔腹主动脉粥样硬化易损斑块中三维应变的影响[J]. 山东大学学报（医学版）, 2017, 55(5): 1-7.

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed