祛毒养心冲剂对病毒性心肌炎的疗效及机制

山东大学学报(医学版)

祛毒养心冲剂对病毒性心肌炎的疗效及机制

王凤^1，2，钟丽红³，周亚滨²,于庆涛¹,唐伟²,雷雪明²,彭莹²,于晗²，董波^1，2

1. 山东大学附属省立医院心内科，济南 250021； 2. 实验畸形学教育部重点实验室
山东大学医学院微生物学教研室，济南 250012；3. 山东大学齐鲁医院药剂科，济南 250012

收稿日期:2007-09-04 修回日期:1900-01-01 出版日期:2008-05-16 发布日期:2008-05-16
通讯作者: 王凤

Effect and mechanism of Quduyangxinchongji on viral myocarditis

WANG Feng ^1，2,　ZHONG Li-hong³, ZHOU Ya-bin², YU Qing-tao¹, TANG Wei²,
LEI Xue-ming², PENG Ying²,　YU Han²,　DONG Bo ^1，2

1. Department of Cardiology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China；2. Key Laboratory of Experimental Teratology， Ministry of Education, China； Department of
Microbiology, School of Medicine, Shandong University, Jinan 250012, China； 3. Department of Pharmaceutics, Qilu Hospital of Shandong University， Jinan 250012， China

Received:2007-09-04 Revised:1900-01-01 Online:2008-05-16 Published:2008-05-16
Contact: WANG Feng

摘要/Abstract

摘要： 目的探讨祛毒养心冲剂对病毒性心肌炎（VMC）的疗效及作用机制。方法将60只小鼠随机分为正常对照组、病毒组、药物组，每组20只。后两组小鼠每只腹腔注射柯萨奇病毒（CVB3）液,正常对照组腹腔注射不含病毒的Eagle′s液。药物组于接种病毒后24h灌胃给予祛毒养心冲剂。21d后，处死所有小鼠并取其心脏。进行心肌病理检查，采用实时荧光定量PCR法和免疫组化技术检测心肌组织中白细胞介素-6（IL-6）mRAN和蛋白的表达。结果与病毒组相比，应用祛毒养心冲剂治疗后，小鼠心肌组织病变积分显著下降（P＜0.05），心肌组织中IL-6的表达明显降低（P＜0.05）。结论祛毒养心冲剂对VMC具有明显治疗作用，其作用机制是通过抑制IL-6在心脏中的表达而实现的。

关键词: BALB C, 祛毒养心冲剂, 白细胞介素-6, 小鼠, 病毒性心肌炎, 柯萨奇病毒B

Abstract: To investigate the effect and mechanism of Quduyangxinchongji on viral myocarditis. MethodsSixty male BABL/C mice were randomly divided into the control group, the virus group and the drug group, with 20 mice in each group. Mice in the second and third groups were intraperitoneally inoculated with coxsackievirus B3, while those in the control group were administrated with Eagle′s without coxsackievirus B3. Also the drug group was subjected to Quduyangxinchongji in the stomach after coxsackievirus B3 infection. All mice were killed 21 days later and their hearts were removed for analysis. The levels of IL-6 mRNA expression and protein in the myocardium were respectively determined by real-time PCR and immunohistochchemistry technique. ResultsAs compared with the virus group, the myocardial histopathological scores and mRNA and protein expressions of IL-6 were significantly decreased in the drug group (P＜0.05). ConclusionQuduyangxinchongji might play a pivotal role in viral myocarditis by inhibiting the IL-6 expression in heart tissues.

Key words: Coxsackievirus, Viral myocarditis, Interleukin-6, Mice, BALB C, Quduyangxinchongji

中图分类号:

R542

王凤,钟丽红,周亚滨,于庆涛,唐伟,雷雪明,彭莹,于晗,董波,. 祛毒养心冲剂对病毒性心肌炎的疗效及机制[J]. 山东大学学报(医学版), 2008, 46(5): 466-469.

WANG Feng ,ZHONG Li-hong,ZHOU Ya-bin,YU Qing-tao,TANG Wei,
LEI Xue-ming,PENG Ying,YU Han,DONG Bo ,. Effect and mechanism of Quduyangxinchongji on viral myocarditis[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2008, 46(5): 466-469.

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