异丙酚对热应激大鼠脑

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (10): 72-74.

异丙酚对热应激大鼠脑

李兴维¹，王端玉²，王志刚¹，赵鑫³，金延武³

山东大学 1. 齐鲁医院麻醉科，济南 250012； 2. 附属济南市中心医院麻醉科；
3. 第二医院麻醉科，济南 250033

收稿日期:2009-01-06 出版日期:2009-10-16 发布日期:2009-10-16
通讯作者: 王志刚（1963- ），男，副主任医师，硕导，主要从事麻醉与镇痛的研究。Email: carefree007@sohu.com
作者简介:李兴维（1984- ），男，硕士研究生，主要从事麻醉与镇痛的研究。E mail: lixingwei7060@sina.com.cn
基金资助:
山东省科学技术发展计划项目资助课题（2007GG20002014

）。

Effect of propofol on betaEodorphin in the brain after 
thermal stress in rats

LI Xingwei¹, WANG Duanyu^2, WANG Zhigang¹, ZHAO Xin3, JIN Yanwu³

1. Department of Analgesia, Qilu Hospital of Shandong University, Jinan 250012, China;
2. Department of Anaesthesiology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China;
3. Department of Analgesia, Second Hospital of Shandong University, Jinan 250033, China

Received:2009-01-06 Online:2009-10-16 Published:2009-10-16

摘要/Abstract

摘要：

目的观察异丙酚对热应激模型大鼠脑组织β内啡肽含量的影响。方法随机将72只雄性Wister大鼠分为对照组(C组，n=8)、热应激模型组(M组，n=32)和异丙酚麻醉高温组(HP组，n=32)；高温组分别于红外线辐射加热后2、6、12、24?h测定大鼠机械痛阈，然后快速取脑，采用放射免疫法测定丘脑β内啡肽含量，采用TUNEL法检测海马神经元凋亡。结果M组和HP组大鼠丘脑β内啡肽含量比C组均明显升高(P<0.05)，机械痛阈也相应升高(P<0.05)；HP组β内啡肽表达在6、12?h时较M组升高(P<0.05)；高温后24?h HP组海马神经元凋亡比M组明显减少(P<0.05)。结论全身高温能增加大鼠丘脑β内啡肽的表达并提高机械痛阈，异丙酚通过减小高温后早期β内啡肽的下降幅度，增强机体热适应能力并发挥抗应激保护作用。

关键词: 二异丙酚, 高温，诱发, &beta, 内啡肽, 疼痛, 细胞凋亡, 大鼠

Abstract:

To investigate the effect and significances of propofol on betaEndorphin in brain tissues after thermal stress in rats. Methods72 male Wister rats were randomly divided into 3 groups. Rats that received neither anesthesia nor WBH treatment served as the control group (group C, n=8), rats which were anesthetized with intraperitoneal injection of chloral hydrate followed with thermal stress served as the model group (group M, n=32), and other rats which were anesthetized with intraperitoneal injection of propofol followed with whole body hyperthermia keeping at 42?℃ for 30?min served as the hyperthermia group (group HP, n=32). The mechanical withdrawal threshold to von Frey hair stimulation was checked at 2, 6, 12 and 24?h after hyperthermia intervention. The rat hypothalamus was then rapidly removed to detect betaEndorphin with the radioimmunoassay method and neuron apoptosis with TUNEL. ResultBetaEndorphin in the hypothalamus of groups M and HP were greatly increased compared with that of the control group, and the mechanical withdrawal threshold to von Frey hair stimulation changed as well (P<0.05). BetaEndorphin of group HP was increased compared with that of group M at 6 and 12 h. The amount of apoptotic neurons increased as follows: group C

Key words: Propofol; Hyperthermia, induced; Betaendorphin; Pain; Apoptosis； Rats

中图分类号:

R456+.1

. 异丙酚对热应激大鼠脑[J]. 山东大学学报(医学版), 2009, 47(10): 72-74.

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