脂肪酸转运蛋白1在多囊卵巢综合征患者颗粒细胞中的表达

doi:10.6040/j.issn.1671-7554.0.2021.1148

摘要/Abstract

摘要： 目的探讨脂肪酸转运蛋白1(FATP1)在多囊卵巢综合征(PCOS)患者颗粒细胞中的表达水平及临床意义。方法选取进行体外受精-胚胎移植(IVF-ET)的75例PCOS患者(PCOS组),根据体质量指数(BMI)≥25判定为肥胖的标准,分为PCOS正常体质量组(n=36)和PCOS肥胖组(n=39);同期62例因输卵管梗阻或男方因素助孕患者(对照组),分为对照正常体质量组(n=31)和对照肥胖组(n=31)。采用逆转录实时定量PCR(qRT-PCR)检测患者颗粒细胞中FATP1的表达,分析其在PCOS患者中的差异性表达。结果 FATP1在PCOS组卵巢颗粒细胞中的表达高于对照组(P <0.001);FATP1在PCOS正常体质量组卵巢颗粒细胞中的表达高于对照正常体质量组(P<0.001); FATP1在PCOS肥胖组卵巢颗粒细胞中的表达高于对照肥胖组(P<0.001)。二分类Logistic回归分析结果显示,高FATP1表达水平(OR=4.242, 95%CI:2.025~8.883, P<0.001)、高抗苗勒氏管激素(AMH,OR=1.512, 95%CI:1.184~1.928, P<0.001)水平、高黄体生成素/卵泡刺激素(LH/FSH,OR=4.301, 95%CI:1.265~15.304, P=0.02)比值和高睾酮(T,OR=1.059, 95%CI:1.003~1.117, P=0.038)水平是影响PCOS的危险因素。结论 FATP1在PCOS患者颗粒细胞中表达增高,可能在PCOS发生发展中发挥重要作用。

关键词: 脂肪酸转运蛋白1, 多囊卵巢综合征, 颗粒细胞, 脂肪酸, 增殖

Abstract: Objective To investigate the expression of fatty acid transport protein 1(FATP1)in granulosa cells of patients with polycystic ovary syndrome(PCOS)and its clinical significance. Methods A total of 75 PCOS patients(PCOS group)undergoing in vitro fertilization-embryo transform(IVF-ET)were selected. According to the standard of body mass index(BMI)≥ 25 as obesity, the subjects were divided into normal BMI PCOS group(n=36)and obese PCOS group(n=39). Another 62 patients(control group)seeking assisted reproduction treatment due to tubal or male factors were divided into normal BMI control group(n=31)and obese control group(n=31). The expression of FATP1 in patients granulosa cells was detected with reverse transcription real-time quantitative PCR(qRT-PCR), and its differential expression in PCOS patients was analyzed. Results The expression of FATP1 was higher in PCOS group than in controls(P<0.001), higher in the normal BMI PCOS group than in normal BMI control group(P<0.001), and higher in obese PCOS group than in obese control group(P<0.001). Binary Logistic regression analysis showed that higher FATP1 level(OR=4.242, 95%CI: 2.025-8.883, P<0.001), higher anti-Mullerian hormone level(AMH, OR=1.512, 95%CI: 1.186-1.928, P<0.001), higher luteotropic hormone/follicle stimulating hormone ratio(LH/FSH, OR=4.401, 95%CI: 1.265-15.304, P=0.02), and higher testosterone level(T, OR=1.059, 95%CI: 1.003-1.117, P=0.038)were risk factors of PCOS. Conclusion The expression of FATP1 is increased in PCOS patients, suggesting that FATP1 may play an important role in the development of PCOS.

Key words: Fatty acid transport protein1, Polycystic ovary syndrome, Granulosa cells, Fatty acid, Proliferation

中图分类号:

R711.75

宋甜,付琳琳,王秋敏,杨晓,王莹,边月红,石玉华. 脂肪酸转运蛋白1在多囊卵巢综合征患者颗粒细胞中的表达[J]. 山东大学学报 (医学版), 2022, 60(2): 22-26.

SONG Tian, FU Linlin, WANG Qiumin, YANG Xiao, WANG Ying, BIAN Yuehong, SHI Yuhua. Expression of fatty acid transport protein 1 in granulosa cells of patients with polycystic ovary syndrome[J]. Journal of Shandong University (Health Sciences), 2022, 60(2): 22-26.

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