自主构建壳聚糖神经导管对大鼠面神经缺损的修复作用

doi:10.6040/j.issn.1671-7554.0.2021.1002

摘要/Abstract

摘要： 目的应用南通大学神经再生重点实验室自主构建的壳聚糖人工神经导管桥接修复模型大鼠面神经缺损,评价其在面神经功能恢复及再生中的作用。方法 24只大鼠分为3组,每组8只,建立大鼠右侧面神经上颊支缺损模型,分别采用自体神经翻转桥接(自体神经组)、壳聚糖神经导管桥接(壳聚糖组)和离断旷置处理(离断组)。运用触须运动评分评估处理后2、4、6、8、10、12周3组大鼠面神经相应支配功能恢复情况,免疫组织化学双染法及透射电镜观察对侧正常神经及处理后12周大鼠造模侧神经的髓鞘与轴突的直径、厚度及层数。结果触须运动评分经广义线性混合效应模型(GLMMs)分析后,分组及时间主效应有统计学意义(P<0.05),分组和时间的交互效应有统计学意义(P<0.05),自体神经组、壳聚糖组分别于处理后4、6周开始出现触须运动功能恢复表现,术后12周自体神经组、壳聚糖组触须运动功能评分差异无统计学意义(P>0.05),离断组触须运动功能无恢复;形态学观察和透射电镜显示自体神经组、壳聚糖组均出现再生神经,自体神经组、壳聚糖组与对侧正常神经三者相比有髓神经纤维直径(F=36.734,P<0.05)、髓壳厚度(F=67.307,P<0.05)、再生髓壳层数(F=75.213,P<0.05)差异有统计学意义;壳聚糖组的有髓神经纤维直径、髓壳厚度和再生髓鞘层数与自体神经组相比差异有统计学意义(P<0.05)。结论动物实验结果表明,该壳聚糖人工神经导管具有促进面神经损伤再生和功能恢复的作用,为临床修复除坐骨神经、桡神经等以外的其他组织周围神经的应用研究提供了依据。

关键词: 壳聚糖, 面神经再生, 损伤, 修复

Abstract: Objective The facial nerve defect of model rats was repaired with a chitosan artificial nerve conduit independently constructed by the Key Laboratory of Nerve Regeneration of Nantong University in order to evaluate its role in facial nerve function recovery and regeneration. Methods A total of 24 rats were divided into 3 groups, with 8 in each group. The defect models of superior buccal branch of right facial nerve were established and treated with autogenous nerve overturning bridging(autogenous nerve group), chitosan nerve conduit bridging(chitosan group)and amputation(amputation group)respectively. The recovery of innervation function of facial nerve was evaluated with whisker motor score at 2, 4, 6, 8, 10 and 12 weeks after treatment. The diameter, thickness and number of layers of myelin sheath and axon of contralateral normal nerve and the facial nerve 12 weeks after treatment were observed with immunohistochemical double staining and transmission electron microscope. Results After the analysis of tentacle motor score with generalized linear mixed effect model(GLMMs), the main effects of grouping and time were statistically significant(P<0.05), and the interaction between grouping and time was statistically significant(P<0.05). The recovery of tentacle motor function began to appear in autologous nerve group and chitosan group at 4 and 6 weeks after treatment, respectively. There was no significant difference between autologous nerve group and chitosan group 12 weeks after operation(P>0.05). There was no recovery of tentacle motor function in amputation group. Morphological observation and transmission electron microscope showed that regenerated nerves were found in autologous nerve group and chitosan group. There were significant differences in the diameter of myelinated nerve fibers(F=36.734, P<0.05), sheath thickness(F=67.307, P<0.05)and the number of regenerated myelin sheath layers(F=75.213, P<0.05)in autologous nerve group, chitosan group and the contralateral normal nerves. The diameter of the myelinated nerve fiber, sheath thickness and the number of regenerated myelin sheath layers in chitosan group were significantly different from those in autologous nerve group(P<0.05). Conclusion The chitosan artificial nerve conduit can promote the regeneration and functional recovery of facial nerve injury, which provides a basis for clinical repair of peripheral nerve besides sciatic nerve and radial nerve.

Key words: Chitosan, Facial nerve regeneration, Injury, Repair

中图分类号:

R651.3

徐菲菲,陈庆文,王东琴,丁必骁,吴昊,杨宇民. 自主构建壳聚糖神经导管对大鼠面神经缺损的修复作用[J]. 山东大学学报 (医学版), 2022, 60(1): 13-20.

XU Feifei, CHEN Qingwen, WANG Dongqin, DING Bixiao, WU Hao, YANG Yumin. Repairing effect of a self-constructed chitosan nerve conduit on facial nerve injury in rats[J]. Journal of Shandong University (Health Sciences), 2022, 60(1): 13-20.

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