硫化氢通过glutaredoxin-1调节氧化应激减轻急性阻塞性睡眠呼吸暂停诱发房颤的机制

doi:10.6040/j.issn.1671-7554.0.2021.0275

摘要/Abstract

摘要： 目的探讨硫化氢(H₂S)减轻急性阻塞性睡眠呼吸暂停(OSA)诱发房颤的机制。方法硫氢化钠(NaHS)作为外源性H₂S供体,8~10周龄健康成年雄性SD大鼠24只,体质量320~360 g,随机分为对照组、OSA诱发房颤组、NaHS+OSA组和氯化镉+NaHS+OSA组,每组6只。按照既往文献构建OSA诱发房颤模型,实验结束后处死大鼠,留取左心耳组织。采用免疫荧光、ELISA法检测左心耳谷氧还蛋白-1的表达水平;采用硫代巴比妥酸法检测左心耳丙二醛(MDA)含量。结果与OSA组相比,NaHS+OSA组房颤诱发次数减少、房颤持续时间缩短(P<0.05);与对照组相比,OSA组左心耳谷氧还蛋白-1表达减少,MDA含量增加,应用NaHS后,NaHS+OSA组谷氧还蛋白-1表达水平较OSA组增加,MDA含量降低(P<0.01),在NaHS基础上应用谷氧还蛋白-1抑制剂氯化镉后,氯化镉+NaHS+OSA组MDA含量增加(P<0.01),H₂S减轻OSA诱发房颤的作用消失。结论外源性H₂S减轻急性OSA诱发房颤,可能是通过上调心肌细胞谷氧还蛋白-1表达从而减轻心肌细胞氧化应激。

关键词: 心房颤动, 阻塞性睡眠呼吸暂停, 硫化氢, 谷氧还蛋白-1, 氧化应激

Abstract: Objective To explore the mechanism of hydrogen sulfide(H₂S)alleviating atrial fibrillation(AF)induced by acute obstructive sleep apnea(OSA). Methods Sodium hydrosulfide(NaHS)was used as exogenous H₂S donor, and 24 healthy adult male SD rats(aged 8-10 weeks)weighing 320 g to 360g were randomly divided into control group, OSA-induced AF group, NaHS+OSA group and cadmium chloride+NaHS+OSA group, with 6 in each group. The model of AF induced by OSA was established according to previous literature. The rats were sacrificed and left atrial appendages were collected. The expression of glutaredoxin-1 was detected with immunofluorescence and ELISA, and the content of malondialdehyde(MDA)was detected with thiobarbituric acid. Results Compared with the OSA group, the NaHS+OSA group had decreased frequency of AF and shortened duration of AF(P<0.05). Compared with control group, the OSA group had decreased expression of glutaredoxin-1 but increased MDA content. After NaHS was used, the expression of glutaredoxin-1 increased but the MDA content decreased compared with the OSA group(P<0.01). After cadmium chloride was used, the MDA content increased(P<0.01), and the effect of H₂S on alleviating OSA-induced AF disappeared. Conclusion Exogenous application of H₂S alleviate acute OSA-induced AF by regulating oxidative stress through glutaredoxin-1.

Key words: Atrial fibrillation, Obstructive sleep apnea, Hydrogen sulfide, Glutaredoxin-1, Oxidative stress

中图分类号:

R541.7

黄辉宁,杜娟娟,孙燚,侯应龙,高梅. 硫化氢通过glutaredoxin-1调节氧化应激减轻急性阻塞性睡眠呼吸暂停诱发房颤的机制[J]. 山东大学学报 (医学版), 2022, 60(1): 1-5.

HUANG Huining, DU Juanjuan, SUN Yi, HOU Yinglong, GAO Mei. Hydrogen sulfide alleviates acute obstructive sleep apnea-induced atrial fibrillation by regulating oxidative stress through glutaredoxin-1[J]. Journal of Shandong University (Health Sciences), 2022, 60(1): 1-5.

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