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山东大学学报 (医学版) ›› 2020, Vol. 58 ›› Issue (6): 76-82.doi: 10.6040/j.issn.1671-7554.0.2020.105

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自噬标志物Beclin-1和P62与增殖因子Ki67在鼻息肉不同部位的表达及其意义

孙文凯1,孙卉2,邬信芳1,高伟3,孟朝暾4,李钦5   

  • 发布日期:2022-09-27
  • 通讯作者: 李钦. E-mail:liqin8226@163.com
  • 基金资助:
    临沂市人民医院研究生培养基金(YJS2019002)

Expressions and clinical significance of Beclin-1, P62 and Ki67 in different parts of nasal polyps

SUN Wenkai1, SUN Hui2, WU Xinfang1, GAO Wei3, MENG Zhaotun4, LI Qin5   

  1. 1. School of clinical Medicine, Binzhou Medical University, Yantai 264000, Shandong, China;
    2. Weifang Clinical Medical College, Weifang Medical University, Weifang 261000, Shandong, China;
    3. Graduate School, Shandong First Medical University, Taian 271000, Shandong, China;
    4. Medical College, Qingdao University, Qingdao 266000, Shandong, China;
    5. Department of Otorhinolaryngology &
    Head and Neck Surgery, Linyi Peoples Hospital, Linyi 276000, Shandong, China
  • Published:2022-09-27

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摘要： 目的 通过检测自噬标志物Beclin-1和P62与增殖因子Ki67在鼻息肉的蒂部及体部的表达及其相关性,探讨自噬与增殖在鼻息肉发展过程中的作用及其机制。 方法 收集2018年7月至2019年5月于临沂市人民医院行手术治疗的46例慢性鼻窦炎伴鼻息肉(CRSwNP)患者的息肉组织作为实验组(分为2个亚组:蒂部组与体部组),另取10例鼻中隔偏曲患者的正常鼻中隔黏膜组织作为对照组。苏木精-伊红(HE)染色并根据鼻息肉组织中嗜酸性粒细胞浸润情况对实验组进行病理分型;免疫组织化学技术法(IHC)检测Beclin-1、P62和Ki67在鼻息肉蒂部、体部及对照组中的蛋白表达情况; Western blotting检测Beclin-1和P62蛋白在3组中的表达;Spearman秩和相关分析法分析鼻息肉组织中Beclin-1、P62和Ki67三者之间的表达相关性。 结果 Beclin-1、P62和Ki67的表达与鼻息肉的病理分型无明显关联(P均>0.05)。Beclin-1在鼻息肉蒂部中的表达强度较体部明显降低,且与对照组比较均降低,差异有统计学意义(H=71.835,P<0.05);P62在鼻息肉蒂部中的表达较体部则明显升高,且与对照组比较均升高,差异有统计学意义 (H=74.666,P<0.05);Ki67在鼻息肉蒂部中的表达较体部则明显升高,且两者与对照组比较均升高,差异有统计学意义(H=76.716,P<0.05)。Beclin-1与P62的表达呈负相关关系(rs=-0.674,P<0.05);Ki67 与Beclin-1表达呈负相关关系(rs=-0.640,P<0.05);Ki67与P62的表达呈正相关关系(rs=0.708,P<0.05)。 结论 自噬标志物Beclin-1和P62与增殖因子Ki67在鼻息肉组织蒂部与体部之间的表达存在差异,并且三者之间的相关性分析表明鼻息肉中自噬与增殖存在负相关关系。鼻息肉蒂部中自噬的活性相对较低而增殖活性相对较高,可能促进了鼻息肉的发生发展。

关键词: 鼻息肉, 自噬, Beclin-1, P62, Ki67, 蒂部, 体部

Abstract: Objective To investigate the role and mechanism of autophagy and proliferation in the development of nasal polyps(NPs)by detecting the expressions and relevance of autophagic markers Beclin-1 and P62 and proliferator Ki67 in the pedicle and body of NPs. Methods NP tissues of 46 patients with chronic rhinosinusitis with nasal polyps(CRSwNP)were collected during July 2018 and May 2019 in Linyi Peoples Hospital as experimental group(pedicle subgroup and body subgroup), and normal nasal septum mucosa of 10 patients with septum deviation were taken as control group. Samples in the experimental group were stained with hematoxylin-eosin(HE)and pathological types were determined based on eosinophil infiltration. The protein expressions of Beclin-1, P62 and Ki67 in the two subgroups and control group were detected with immunohistochemistry(IHC). The expressions of Beclin-1 and P62 were detected with Western blotting. The correlation among Beclin-1, P62 and Ki67 was analyzed with Spearman rank correlation assay. Results There was no significant association between expressions of Beclin-1, P62 and Ki67 and pathological types of NPs(P>0.05). Beclin-1 expression was significantly lower in pedicle subgroup than in body subgroup, and was lower than that in the control group(H=71.835, P<0.05). P62 expression was significantly higher in pedicle subgroup than in body subgroup, and was higher than that in the control group(H=74.666, P<0.05). Ki67 expression was significantly higher in pedicle subgroup than in body subgroup, and was higher than that in the control group(H=76.716, P<0.05). Beclin-1 was negatively correlated with P62(rs=-0.674, P<0.05). Ki67 was negatively correlated with Beclin-1(rs=-0.640, P<0.05)but positively correlated with P62(rs=0.708, P<0.05). Conclusion Expressions of Beclin-1, P62 and Ki67 are different between pedicle and body in NPs, and there is negative correlation between Beclin-1, P62 and Ki67. The expressions of Beclin-1 and P62 are lower while expression of Ki67 is higher in pedicle than in body, indicating that Ki67 may promote the occurrence and development of NPs.

Key words: Nasal polyps, Autophagy, Beclin-1, P62, Ki67, Pedicle of nasal polyps, Body of nasal polyps

中图分类号: 

  • R765
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