高糖、高脂饮食与不同浓度硒对大鼠脂代谢及氧化应激的影响

doi:10.6040/j.issn.1671-7554.0.2019.1394

摘要/Abstract

摘要： 目的观察高糖、高脂饮食对大鼠脂代谢及氧化应激的影响以及不同膳食模式下添加不同浓度硒的效果差异。方法使用亚硒酸钠调节饲料中硒浓度,并根据饲料将90只大鼠随机分为9组,喂养16周,测定各组大鼠体质量,计算脏器系数,测定血清甘油三酯、总胆固醇、脂肪酶和硒浓度,制备肝脏、心脏匀浆后测定谷胱甘肽过氧化物酶、丙二醛、总超氧化物歧化酶、铜锌超氧化物歧化酶, 观察肝脏及心脏病理变化。结果高糖饮食大鼠甘油三酯及丙二醛、高脂饮食大鼠总胆固醇及丙二醛均高于普通饮食大鼠(P<0.05);普通中硒组大鼠的血清甘油三酯,普通高硒组心脏谷胱甘肽过氧化物酶均低于普通低硒组(P<0.05);高糖中硒组与高糖低硒组大鼠相比,肝脏谷胱甘肽过氧化物酶升高、心脏丙二醛降低(P<0.05);高脂高硒组较高脂低、中组血清甘油三酯及心脏丙二醛升高,高脂中硒组与高脂低硒组相比肝脏丙二醛下降(P<0.05);高糖高硒组、高脂高硒组血硒升高(P<0.05)。结论适当补充硒对大鼠脂代谢及抗氧化作用有保护作用,性别因素可能影响高浓度硒对高糖、高脂大鼠的作用效果,具体机制有待进一步讨论。

关键词: 膳食模式, 高糖饮食, 高脂饮食, 硒, 脂代谢, 氧化应激

Abstract: Objective To observe the effects of high-sugar and high-fat diet on lipid metabolism and oxidative stress in rats and the effects of different selenium concentrations in different diets. Methods Sodium selenite was used to adjust the selenium concentration in the feed, and 90 rats were randomly divided into 9 groups and fed for 16 weeks. The body weight was measured, organ coefficient was calculated, and serum triglycerides(TG), total cholesterol(TC), concentrations of lipase and selenium were measured. After preparation of liver and heart homogenates, glutathione peroxidase(GPx), malondialdehyde(MDA), total superoxide dismutase(T-SOD), and copper-zinc superoxide dismutase(CuZu-SOD)were measured. Pathological changes of liver and heart were observed. Results High-sugar rats had higher TG and MDA, and high-fat rats had higher TC and MDA than normal-diet rats(P<0.05). Normal-medium-selenium rats had lower serum TG, and normal-high-selenium rats had lower cardiac GPx than normal-low-selenium rats(P<0.05). High-sugar-medium-selenium rats had higher hepatic GPx but lower cardiac MDA than high-sugar-low-selenium rats(P<0.05). High-fat-high-selenium rats had higher serum TG and cardiac MDA than high-fat-medium-selenium rats and high-fat-low-selenium rats(P<0.05). High-fat-medium-selenium rats had lower hepatic MDA than high-fat-low-selenium rats(P<0.05). High-glucose-high-selenium rats and high-fat-high-selenium rats had increased serum selenium(P<0.05). Conclusion Appropriate selenium supplementation has a protective effect on lipid metabolism and antioxidation in rats. Gender may affect the effect of high concentrations of selenium on high-glucose and high-fat rats. The mechanism remains to be explored.

Key words: Dietary pattern, High-sugar diet, High-fat diet, Selenium, Lipid metabolism, Oxidative stress

中图分类号:

李艳,孙凤娇,张天然,王雨心,张正铎,高希宝. 高糖、高脂饮食与不同浓度硒对大鼠脂代谢及氧化应激的影响[J]. 山东大学学报 (医学版), 2020, 58(5): 98-106.

LI Yan, SUN Fengjiao, ZHANG Tianran, WANG Yuxin, ZHANG Zhengduo, GAO Xibao. Effects of high-sugar, high-fat diet and different concentrations of selenium on lipid metabolism and oxidative stress in rats[J]. Journal of Shandong University (Health Sciences), 2020, 58(5): 98-106.

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