大蒜素后处理对大鼠心肌缺血-再灌注损伤的保护作用

doi:10.6040/j.issn.1671-7554.0.2017.089

摘要/Abstract

摘要： 目的探讨大蒜素后处理对心肌缺血-再灌注损伤的作用。方法 18只大鼠随机分为缺血再灌注(IR)组、缺血后处理(IPO)组和大蒜素后处理组,每组6只。采用离体心脏Langendorff灌注模型,各组平衡灌注20 min后,停灌30 min,复灌60 min。记录平衡灌注末及再灌结束时的左心室发展压(LVDP)、左室内压上升最大速率(+dp/dt_max)、左室内压下降最大速率(-dp/dt_max)和心率(HR)。检测平衡灌注末及再灌结束时心脏灌流液中乳酸脱氢酶(LDH)的活性;灌注结束后检测左心室心肌组织中超氧化物歧化酶(SOD)活力和丙二醛(MDA)含量。结果平衡灌注末各组间心功能指标差异无统计学意义(P>0.05)。与IR组比较,再灌注60 min时大蒜素后处理组能明显改善心功能各项指标,心脏灌流液中LDH的活性降低,心肌组织中SOD活力提高,MDA含量降低,差异均有统计学意义(P<0.05)。与IPO组比较,再灌注60 min时大蒜素后处理组HR减慢,差异有统计学意义(P<0.001);其他各项指标差异无统计学意义(P>0.05)。结论再灌注开始给予适量大蒜素能够降低缺血再灌注引起的心肌损伤,改善心功能,其作用与IPO效应相当。

关键词: 缺血后处理, 心肌, 大蒜素后处理, 缺血-再灌注损伤, 大鼠

Abstract: Objective To explore the effects of allicin postconditioning on myocardial ischemia-reperfusion injury in rats. Methods A total of 18 Wistar rats were randomly allocated into 3 groups: ischemia reperfusion group(IR group), ischemic postconditioning group(IPO group)and allicin postconditioning group, with 6 rats in each group. Langendorff perfusion apparatus was used to build an isolated rat myocardial IR model. Isolated rat hearts were subjected to 30 min global ischemia followed by 60 min reperfusion after 20 min equilibrium. The left ventricular develop pressure(LVDP), the maximal ventricular pressure rise ratio during systolic period(+dp/dt_max), the maximal ventricular pressure decrease ratio during diastolic period(-dp/dt_max)and heart rate(HR)were recorded at the end of 20 min equilibrium and 60 min reperfusion separately. Heart perfusion transudate at the end of 20 min equilibrium and 60 min reperfusion were assessed to determine the activity of lactate dehydrogenase(LDH). Myocardium of left ventricle was detected to examine the activity of superoxide dismudase(SOD)and the contents of malondialdehyde(MDA). Results There was no difference in equilibrium hemodynamics observed among the groups(P>0.05). At the end of reperfusion, compared with IR group, the allicin postconditioning group showed significantly ameliorated functional recovery, 山东大学学报 (医学版)55卷7期 -刘芳,等.大蒜素后处理对大鼠心肌缺血-再灌注损伤的保护作用 \=-decreased activity of LDH, enhanced activity of SOD, and decreased contents of MDA(all P<0.05). At the end of reperfusion, HR was obviously slower in the allicin postconditioning group than in the IPO group(P<0.001). There was no statistically significant difference between IPO group and allicin postconditioning group in other indicators(P>0.05). Conclusion Allicin administration at the beginning of reperfusion can significantly reduce ischemia-reperfusion injury and improve cardiac function, with similar cardioprotective effect as IPO.

Key words: Allicin postconditioning, Ischemic postconditioning, Myocardium, Rat, Ischemia-reperfusion injury

中图分类号:

R654.2

刘芳,乔蕾,陈文超,杨丽娜. 大蒜素后处理对大鼠心肌缺血-再灌注损伤的保护作用[J]. 山东大学学报（医学版）, 2017, 55(7): 6-11.

LIU Fang, QIAO Lei, CHEN Wenchao, YANG Lina. Cardioprotective effects of allicin postconditioning on myocardial ischemia-reperfusion injury in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2017, 55(7): 6-11.

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