富半胱氨酸蛋白61在高脂诱导肥胖小鼠脂肪组织中的表达

doi:10.6040/j.issn.1671-7554.0.2017.150

摘要/Abstract

摘要： 目的探讨富半胱氨酸蛋白61(Cyr61)在肥胖小鼠脂肪组织中的表达、分布及其与脂肪慢性炎症的关系。方法 4周龄雄性C57BL/6小鼠30只,随机分为普通饮食组(NC组)和高脂喂养组(HFD组),每组15只。HFD组给予60%脂肪含量高脂饲料喂养,构建肥胖小鼠模型,NC组给予普通饲料喂养。造模成功后测量小鼠体脂含量,通过在腹腔注射葡萄糖进行葡萄糖耐量试验(IPGTT)检测糖耐量和血脂,取睾周脂肪检测Cyr61的表达及分布,并检测脂肪组织肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的mRNA表达量。结果 HFD组脂质量、体内脂肪含量明显增加,糖耐量减低,血清总胆固醇和低密度脂蛋白胆固醇较正常高(P<0.05)。HFD组脂肪组织TNF-α、IL-6表达上调(P<0.05),Cyr61在HFD组脂肪组织中蛋白及mRNA水平均增加(P<0.05),且主要分布于脂肪组织基质中。结论 Cyr61在肥胖小鼠脂肪组织表达明显增加,主要定位于脂肪组织基质中,且与炎症因子表达具有一致性,提示其可能与慢性炎症的发生相关。

关键词: 富半胱氨酸蛋白61, 脂肪组织, 肥胖, 炎症因子, 慢性炎症

Abstract: Objective To investigate the expression and distribution of cysteine rich protein 61(Cyr61)in the adipose tissue of obese mice and its relationship with fat chronic inflammation. Methods A total of 30 male C57BL/6 mice were randomly divided into the normal diet group(NC group)and high fat diet group(HFD group). The HFD group was fed with high fat diet of 60% fat content to construct the obese mouse models. The NC group was fed with normal diet. After modeling, the body fat content of mice was measured, glucose tolerance and blood lipids were detected with intraperitoneal injection of glucose tolerance test(IPGTT), the expression and distribution of Cyr61 in the testis tissue were measured, and the expressions of tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)mRNA in the adipose tissue were detected. Results The body weight, body fat content and fat tolerance of obese mice were significantly higher than those in NC group(P<0.05). The serum cholesterol and low density lipoprotein cholesterol were significantly higher than those in NC group(P<0.05). The expressions of TNF-α and IL-6 in the adipose tissue of obese mice were up-regulated(P<0.05). Both of the protein and mRNA levels of Cyr61 increased in the adipose tissue of HFD group(P<0.05), and Cyr61 was mainly distributed in the adipose tissue matrix. Conclusion Cyr61 expression, which is mainly located in the adipose tissue matrix, is significantly increased in the adipose tissue of obese mice, and is consistent with the expression of inflammatory factors, suggesting that it may be related to the occurrence of chronic inflammation.

Key words: Cysteine rich protein, Adipose tissue, Obesity, Inflammatory factor, Chronic inflammation

中图分类号:

R723.14

卿立,何天祎,乔婧婷,崔晨,侯新国,陈丽. 富半胱氨酸蛋白61在高脂诱导肥胖小鼠脂肪组织中的表达[J]. 山东大学学报 (医学版), 2017, 55(12): 7-12.

QING Li, HE Tianyi, QIAO Jingting, CUI Chen, HOU Xinguo, CHEN Li. Expression of Cyr61 in the adipose tissue of obese mice induced by high fat diet[J]. Journal of Shandong University (Health Sciences), 2017, 55(12): 7-12.

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