红景天苷对人胃癌细胞SGC-7901抑瘤作用的体外实验研究

doi:10.6040/j.issn.1671-7554.0.2015.131

摘要/Abstract

摘要： 目的研究红景天苷对体外培养的人胃癌细胞SGC-7901增殖及生长的影响,并探讨其抗肿瘤作用的可能机制。方法实验分为对照组、0.50 mg/mL红景天苷组、1.00 mg/mL红景天苷组及2.00 mg/mL红景天苷组。通过MTT比色法、核仁组成区嗜银蛋白(AgNOR)染色、流式细胞技术、细胞划痕实验的方法检测不同浓度红景天苷对SGC-7901细胞增殖、AgNOR表达、细胞周期、细胞凋亡及细胞迁移作用的影响。结果与对照组相比,不同浓度的红景天苷组SGC-7901细胞增殖及AgNOR表达均受到明显抑制(P<0.05),结果呈明显的浓度-时间-效应依赖关系;细胞停滞在S期的比例减少、G₂/M期的比例增加(P<0.05);细胞凋亡率升高,迁移率降低(P<0.05)。结论红景天苷能抑制SGC-7901细胞增殖,并能阻滞细胞周期和诱导细胞凋亡,降低胃癌细胞的迁移能力。

关键词: 红景天苷, 胃癌细胞, 增殖, 抗肿瘤

Abstract: Objective To investigate the effect of salidroside on the proliferation and growth of in vitro cultured human gastric carcinoma SGC-7901 cells, and to explore the possible anti-tumor mechanism. Methods Cells were divided into control group, 0.50 mg/mL salidroside group, 1.00 mg/mL salidroside group and 2.00 mg/mL salidroside group. Cell proliferation was assessed by MTT array. Argyrophilic nucleolar organiser region proteins (AgNOR) staining was used to determine the expression of AgNOR in nuclear. The cell cycle and apoptosis were detected by flow cytometry test. Migration rate was measured by cell scratch assay. Results Compared with control group, the proliferation and AgNOR expression of SGC-7901 cells were inhibited significantly in salidroside groups (P<0.05) with a concentration-, time-and effect-depent manner; cell circle arrest increased at G₂/M phase, but decreased at S phase (P<0.05); the rate of cell apoptosis increased, while cell migration rate was down-regulated (P<0.05). Conclusion Salidroside can effectively inhibit proliferation, arrest cell cycle, induce apoptosis and decrease migration rate of SGC-7901 cell.

Key words: Gastric carcinoma cells, Proliferation, Salidroside, Anti-tumor

中图分类号:

R331

韦丽兰, 莫书荣. 红景天苷对人胃癌细胞SGC-7901抑瘤作用的体外实验研究[J]. 山东大学学报（医学版）, 2015, 53(12): 12-15,32.

WEI Lilan, MO Shurong. An in vitro study of the anticancer effect of salidroside on human gastric carcinoma SGC-7901 cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2015, 53(12): 12-15,32.

参考文献

[1] 季加孚.我国胃癌防治研究三十年回顾[J].中国肿瘤临床, 2013, 40(22):1346-1351.
[2] 吴菲,林国桢,张晋昕.我国恶性肿瘤发病现状及趋势[J].中国肿瘤, 2012, 21(2):81-85.
[3] 宋汉君,吕少春,李丽疆,等.红景天苷的抗肿瘤作用[J].中国老年学杂志, 2011, 31(20):3991-3992.
[4] Noreen EE, Buckley JG, Lewis SL, et al. The effects of an acute dose of Rhodiola rosea on endurance exercise performance[J]. J Strength Cond Res, 2013, 27(3):839-847.
[5] Getova DP, Mihaylova AS. Effects of Rhodiola rosea extract on passive avoidance tests in rats[J]. Cent Eur J Med, 2013, 8(2):176-181.
[6] Udintsev SN, Shakhov VP. The role of humoral factors of regenerating liver in the development of experimental tumors and the effect of Rhodiola rosea extract on this process[J]. Neoplasma, 1991, 38(3):323-331.
[7] 许朝晖,王永才,张蕾,等.嗜银蛋白(AgNOR)对针吸脱落细胞癌肿早期诊断研究[J].中国实验诊断学, 2010, 14(12):2034-2035.
[8] 韩益玲,钟璐,陈芳源,等.急性非淋巴细胞白血病细胞核仁组织区相关嗜银蛋白的研究[J].上海交通大学学报:医学版, 2009, 29(1):73-75.HAN Yiling, ZHONG Lu, CHEN Fangyuan, et al. Study of argyrophilic nucleola organizer region proteins in acute myeloid leukemia cells[J]. Joumal of Shanghai Jiaotong University:Medical Science, 2009, 29(1):73-75.
[9] 李冰.温度和时间对核仁组成区嗜银蛋白染色结果的影响[J].齐齐哈尔医学院学报, 2010, 31(15):2433-2434.
[10] 曹蕊,吴雄志.中药对肿瘤细胞周期影响的研究进展[J].中国肿瘤临床, 2012, 39(10):749-752.
[11] 魏向阳,涂悦,徐忠伟,等.紫杉醇对U373细胞周期阻滞及增殖抑制的机制研究[J].中国药理学通报, 2011, 27(4):543-547. WEI Xiangyang, TU Yue, XU Zhongwei, et al. The mechanism of the induction of cell cycle arrest and inhibition on U373 cells by PTX[J]. Chinese Pharmacological Bulletin, 2011, 27(4):543-547.
[12] Feng Y, Dai X, Li X, et al. EGF signaling pathway regulates colon cancer stem cell proliferation and apoptosis[J]. Cell Prolif, 2012, 45(5):413-419.
[13] Burikhanov R, Shrestha-Bhattarai T, Qiu S, et al. Novel mechanism of apoptosis resistance in cancer mediated by extracellular PAR-4[J]. Cancer Res, 2013, 73(2):1011-1019.
[14] 何莉莎,李枋霏,孙桂芝,等.中药诱导肿瘤细胞凋亡的实验研究进展[J].河北中医, 2014, 36(2):309-311.
[15] 张晔,赵明芳,曲秀娟,等.β-榄香烯联合紫杉醇诱导胃癌细胞凋亡的体外研究[J].现代肿瘤医学, 2013, 21(5):965-968. ZHANG Ye, ZHAO Mingfang, QU Xiujuan, et al. β-elemene combined with paclitaxel induced cell apoptosis in human gastric cancer cells[J]. Modern Oncology, 2013, 21(5):965-968.
[16] 宋文瑛,许冠华,张光霁.猕猴桃根多糖对人胃癌SGC-7901细胞增殖凋亡及P-P38表达的影响[J].中国中西医结合杂志, 2014, 34(3):329-333.
[17] 马勇,吴新民,郭亚民.胃癌转移相关分子标志物的研究进展[J].现代肿瘤医学, 2014, 22(12):3017-3019. MA Yong, WU Xinmin, GUO Yamin. Research progress of molecular markers in metastasis gastric carcinoma[J]. Modern Oncology, 2014, 22(12):3017-3019.
[18] Kobayashi H, Boelte KC, Lin PC. Endothelial cell adhesion molecules and cancer progression[J]. Curr Med Chem, 2007, 14(4):377-386.
[19] 涂祥俊,邓述恺.肿瘤转移的分子机制[J].肿瘤基础与临床, 2013, 26(3):269-272.
[20] Zlotnik A, Burkhardt AM, Homey B. Homeostatic chemokine receptors and organ-specific metastasis[J]. Nat Rev Immunol, 2011, 11(9):597-606. (编辑:相峰)

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