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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (12): 7-11.doi: 10.6040/j.issn.1671-7554.0.2015.025

• 基础医学 • 上一篇    下一篇

酪酪肽对胰腺癌Miapaca-2细胞凋亡的影响

李红霞1, 董蕾2, 姜炅2, 王新阳3   

  1. 1. 西安交通大学第一附属医院消化内科, 陕西西安 710061;
    2. 西安交通大学第二附属医院消化内科, 陕西西安 710004;
    3. 西安交通大学第一附属医院泌尿外科实验室, 陕西西安 710061
  • 收稿日期:2015-01-08 出版日期:2015-12-10 发布日期:2015-12-10
  • 通讯作者: 李红霞。E-mail:hongxia1105@126.com E-mail:hongxia1105@126.com
  • 基金资助:
    国家自然科学基金(81170352)

Effects of peptide YY on apoptosis of human pancreatic cancer cell line Miapaca-2 in vitro

LI Hongxia1, DONG Lei2, JIANG Jiong2, WANG Xinyang3   

  1. 1. Department of Gastroenterology, the First Affiliated Hostipal of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China;
    2. Department of Gastroenterology, the Second Affiliated Hostipal of Xi'an Jiaotong University, Xi'an 710004, Shaanxi, China;
    3. Urological Research Laboratory, the First Affiliated Hostipal of Xi'an Jiaotong University, Xi'an 710061, Shaanxi, China
  • Received:2015-01-08 Online:2015-12-10 Published:2015-12-10

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摘要： 目的 观察酪酪肽(PYY)对体外培养的胰腺癌Miapaca-2细胞凋亡的影响。方法 不同浓度(0.01、0.005、0.0025、0.00125 mmol/L) PYY分别作用于体外常规培养的Miapaca-2细胞48 h后,流式细胞仪检测细胞凋亡率、细胞周期;RT-PCR法检测Survivin mRNA,Western blotting法检测Survivin、Caspase-3蛋白的表达。结果 PYY处理48 h后细胞凋亡率明显升高,并呈浓度依赖性;G0/G1期细胞数明显增多,S、G2/M期细胞数明显减少,G1期前出现亚二倍体凋亡峰;PYY明显抑制Survivin蛋白和mRNA表达(P<0.01),上调Caspase-3蛋白表达(P<0.01)。结论 PYY能诱导胰腺癌Miapaca-2细胞凋亡,参与细胞周期调控,其机制可能与抑制Survivin蛋白和mRNA表达、上调Caspase-3蛋白表达有关。

关键词: Caspase, 酪酪肽, 胰腺癌细胞, 细胞凋亡, Survivin

Abstract: Objectives To observe the effects of peptide YY(PYY)on inducing apoptosis of human pancreatic cancer cell line Miapaca-2. Methods The pancreatic cancer cell line Miapaca-2 was treated with PYY of different concentrations(0.01, 0.005, 0.0025 and 0.00125 mmol/L) for 48 h.The apoptosis and cell cycle were evaluated by flow cytometry, the expression of survivin mRNA was detected with RT-PCR, and the expressions of survivin and Caspase-3 protein were detected with Western blotting. Results After intervention with PYY for 48 h, celluar apoptosis rate was significantly increased in a dose-dependent manner. The cells of G0/G1 stage were significantly increased, but the cells of S and G2/M stages were significantly decreased. The hypodiploid apoptotic peak before the G1 stage was detected. PYY inhibited survivin protein and survivin mRNA expressions but increased Caspase-3 protein expression notably in a dose dependent manner(P<0.01). Conclusion PYY can induce apoptosis in a dose-dependent manner. PYY maybe block cell cyclein G0/G1 stage, downregulate survivin mRNA and protein expressions, increase Caspase-3 protein expression and finally induce apoptosis.

Key words: Peptide YY, Apoptosis, Pancreatic cancer cell, Survivin, Caspase

中图分类号: 

  • R735.9
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