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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (7): 32-36.doi: 10.6040/j.issn.1671-7554.0.2013.702

• 基础医学 • 上一篇    下一篇

负载凋亡肿瘤细胞的树突状细胞对喉癌细胞的杀伤作用

张敏1, 王雪峰2   

  1. 1. 辽宁医学院, 辽宁 锦州 121001;
    2. 辽宁医学院附属第一医院耳鼻咽喉头颈外科, 辽宁 锦州 121001
  • 收稿日期:2013-11-23 修回日期:2014-06-10 出版日期:2014-07-10 发布日期:2014-07-10
  • 通讯作者: 王雪峰。E-mail：jzswwang@126.com E-mail:jzswwang@126.com
  • 基金资助:
    辽宁省科学技术计划项目（2012225019）

Killing effect of apoptotic-tumor-cell-loaded dendritic cells on laryngeal carcinoma cells

ZHANG Min1, WANG Xuefeng2   

  1. 1. Liaoning Medical University, Jinzhou 121001, Liaoning, China;
    2. Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning, China
  • Received:2013-11-23 Revised:2014-06-10 Online:2014-07-10 Published:2014-07-10

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摘要： 目的 探讨凋亡肿瘤细胞抗原负载的树突状细胞（DC）对喉癌细胞杀伤作用的影响。方法 用细胞因子重组人白细胞介素4（rhIL-4）、重组人粒细胞巨噬细胞集落刺激因子（rhGM-CSF）与肿瘤坏死因子α（rhTNF-α）体外诱导扩增人外周血单个核来源的DC，6 d后将DC分为凋亡组、冻融组和未处理组；各组细胞继续培养4 h后，分别采用流式细胞术检测DC表面分子的表达、ELISA法检测干扰素γ（IFN-γ）的水平、MTT法评估各组DC刺激细胞毒性T淋巴细胞（CTL）增殖的能力及诱导CTL的杀伤活性。结果 与其余各组相比，凋亡组DC表面分子与IFN-γ的表达明显增加（P<0.05），CTL的增殖能力与杀伤活性增强（P<0.05）。结论 凋亡肿瘤细胞可以促进DC的分化与成熟，并能使DC产生高效而特异性的抗喉癌免疫应答。

关键词: 树突状细胞, 凋亡肿瘤细胞, 喉癌细胞, 顺铂

Abstract: Objective To explore the killing effect of apoptotic-tumor-cell-loaded dendritic cells (DCs) on the laryngeal carcinoma cells. Methods DCs from peripheral blood monocytes were induced by recombinant human interleukin-4 (rhIL-4), recombinant human granulocyte/macrophage colony-stimulating factor (rhGM-CSF) and tumor necrosis factor (TNF-α) in vitro, then were divided into the apoptotic laryngeal carcinoma cells group, freeze-thaw cells group and untreated cells group 6 days later. After continuous culture of DCs for 4h, the expressions of the surface markers of DCs were detected by flow cytometry, the level of Interferon-γ (IFN-γ) was measured by ELISA, and the proliferation ability and killing effect of cytotoxic T lymphocyte (CTL) stimulated by DCs were evaluated by MTT. Results Compared with other groups, apoptotic laryngeal carcinoma cells group could enhance the expression of the surface markers CD83, CD86, HLA-DR and IFN-γ(P<0.05), and the proliferation ability and killing effect of CTL increased (P<0.05). Conclusion Apoptotic carcinoma cells can promote the differentiation and maturation of DCs, and induce effective and specific immune response against laryngeal carcinoma.

Key words: Dendritic cell, Laryngeal carcinoma cell, Cisplatin, Apoptotic carcinoma cells

中图分类号: 

  • R730.51
[1] Gao C, Pan J, Lu W, et al. In-vitro evaluation of paclitaxel-loaded MPEC-PLGA nanoparticles on laryngeal carcinoma cells[J]. Anticancer Drugs, 2009, 20(9):807-814.
[2] Ueno H, Klechevsky E, Morita R, et al. Dendritic cell subsets in health and disease[J]. Immunol Rev, 2007, 219:118-142.
[3] SU X, Zhang L, Jin L, et al. Coculturing dendritic cells with zoledronate acid efficiently enhance the anti-tumor effect of cytokine-induced killer cells[J]. J Clin Immunol, 2010, 30(5):766-774.
[4] 冯勤梅,吴霞,王颖,等.紫杉醇和顺铂诱导下的凋亡卵巢癌细胞对树突状细胞提呈功能的影响[J].细胞与分子免疫学杂志, 2009, 25(1):38-41.
[5] Koski G K, Cohen P A, Roses R E, et al. Reengineering dendritic cell-based anti-cancer vaccines[J]. Immunological Rev, 2008, 222:256-276.
[6] Yu X, Xia W, Zhang T, et al. Enhanced cytotoxicity of IL-24 gene-modified dendritic cells co-cultured with cytokine-induced killer cells to hepatocellular carcinoma cells[J].Int J Hematol, 2010, 92(2):276-282.
[7] Ellebaek E, Engell-Noerregaard L, Iversen T Z, et al. Metastatic melanoma patients treated with dendritic cell vaccination, Interleukin-2 and metronomic cyclophosphamide:results from a phase II trial[J].Cancer Immunol Immunother, 2012, 61(10):1791-1804.
[8] Nasr N, Harman A, Turville S, et al. HIV infection of dendritic cells[J]. Methods Mol Biol, 2014, 1087:221-232.
[9] Gregori S. Dendritic cells in networks of immunological tolerance[J]. Tissue Antigens, 2010, 77(2):89-99.
[10] Pinzon-Charry A, Ho C S, Maxwe T, et al. Numerical and functional defects of blood dendritic cells in early- and late-stage breast cancer[J]. Br J Cancer, 2007, 97(9):1251-1259.
[11] Diwan M, Elamanchili P, Lane H, et al. Biodegradable nanoparticle mediated antigen delivery to human cord blood derived dendritic cells for induction of primary T cell responses[J]. J Drug Target, 2003, 11(8-10):495-507.
[12] Sanecka A, Ansems M, Prosser A C, et al. DC-STAMP knock-down deregulates cytokine production and T-cell stimulatory capacity of LPS-matured dendritic cell[J]. BMC Immunology, 2011, 12:57. doi:10.1186/1471-2172-12-57.
[13] Zhong R, Teng J, Han B, et al. Dendritic cells combining with cytokine-induced killer cells synergize chemotherapy in patients with late-stage non-small cell lung cancer[J]. Cancer Immunol Immunother, 2011, 60(10):1497-1502.
[14] Negroni L, Samson M, Guigonis J M, et al. Treatment of colon cancer cells using the cytosine deaminase/5-fluorocytosine suicide system induces apoptosis, modulation of the proteome, and Hsp90beta phosphorylation[J]. Mol Cancer Ther, 2007, 10:2747-2756.
[15] Gottfried-Blackmore A, Kaunzner U W, Idoyaga J, et al. Acute in vivo exposure to interferon-γ enables resident brain dendritic cells to become effective antigen presenting cells[J]. Proc Natl Acad Sci U S A, 2009, 106(49):20918-20923.
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