熊果酸体内抗肺癌作用机制探讨

山东大学学报(医学版)

熊果酸体内抗肺癌作用机制探讨

李希波，孙雪飞，尹秋伟，王德江

山东大学齐鲁医院胸外科，山东济南 250021

收稿日期:2006-12-20 修回日期:1900-01-01 出版日期:2007-02-24 发布日期:2007-02-24
通讯作者: 李希波

Ursolic acid inhibits the angiogenesis of transplanted human lung adenocarcinoma cell line A549 in nude mice

LI Xi-bo, SUN Xue-fei, YIN Qiu-wei, WANG De-jiang

Department of Thoracic Surgery, Qilu Hospital, Shandong University, Jinan 250012, Shandong, China

Received:2006-12-20 Revised:1900-01-01 Online:2007-02-24 Published:2007-02-24
Contact: LI Xi-bo

摘要/Abstract

摘要： 目的：探讨熊果酸对人肺癌裸鼠移植瘤的抑制作用及其机制。方法:采用人肺腺癌A549细胞株进行裸鼠移植瘤实验。通过移植瘤生长曲线、终末瘤重计算抑制率观察熊果酸对人肺癌裸鼠移植瘤的抑制作用，采用免疫组化法检测VEGF蛋白表达、微血管密度，TUNEL检测凋亡指数，探讨其作用机制。结果:实验组用药后移植瘤生长缓慢，实验结束时，实验组瘤重及瘤体积明显低于对照组（P＜0.05），抑瘤率为51.35％。实验组移植瘤组织微血管密度及VEGF蛋白表达明显低于对照组（P均＜0.01），实验组癌细胞凋亡指数高于对照组(P＜0.01)。结论:熊果酸对裸鼠肺腺癌移植瘤生长有抑制作用，其机制可能与降低移植瘤促血管生成因子VEGF的表达、抑制新生血管的形成和促进肿瘤细胞凋亡有关。

Abstract: Objective： To study the inhibitory effect of ursolic acid on the transplanted human lung adenocarcinoma cell line A549 in nude mice and explore its mechanism. Methods： The nude mice bearing A549 cancer cells were established in vivo. The transplanted tumor growth curve was drawn, and the inhibitive ratio was calculated according to tumor weight. Immunohistochemistry (S-P)was used to examine the expression of VEGF and MVD of the transplanted tumor, and TUNEL (Terminal deoxynucleotidyl transferase mediated UTP nick end labeling) was used to examine the apoptosis index of the transplanted tumor. Results： The transplanted tumor of the treated group grew slowly. The volume and the weight of tumors in the treated group were smaller than those in the control group. The tumor inhibitive rate was 51.35％. The expressions of VEGF and MVD in the treated group were lower than those of the control group, and the apoptosis index of the transplanted tumor in the treated group was higher than that in the control group(P＜0.01). Conclusion： Ursolic acid has an anti-angiogenesis activity on the human lung adenocarcinoma cell line A549 in vivo by inducing the apoptosis of the cancer cells, deceasing the VEGF expression and inhibiting the vasoformation of the transplanted tumor.

Key words: Lung cancer, Apoptosis, Angiogenesis, Ursolic acid

中图分类号:

R734.2

李希波,孙雪飞,尹秋伟,王德江. 熊果酸体内抗肺癌作用机制探讨[J]. 山东大学学报(医学版), 2007, 45(2): 188-191.

LI Xi-bo,SUN Xue-fei,YIN Qiu-wei,WANG De-jiang. Ursolic acid inhibits the angiogenesis of transplanted human lung adenocarcinoma cell line A549 in nude mice[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(2): 188-191.

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