毛果芸香碱滴眼对兔眼角膜内皮细胞凋亡作用的影响

山东大学学报(医学版)

毛果芸香碱滴眼对兔眼角膜内皮细胞凋亡作用的影响

陶远,王红,乔智

山东大学齐鲁医院眼科，济南 250012

收稿日期:2007-09-30 修回日期:1900-01-01 出版日期:2007-12-24 发布日期:2007-12-24
通讯作者: 乔智

Effect of pilocarpine on apoptosis of corneal endothelium in rabbits

TAO Yuan， WANG Hong， QIAO Zhi

Department of Ophthalmology, Qilu Hospital of Shandong University, Jinan 250012, China

Received:2007-09-30 Revised:1900-01-01 Online:2007-12-24 Published:2007-12-24
Contact: QIAO Zhi

摘要/Abstract

摘要： 目的探讨长期应用毛果芸香碱对兔眼角膜内皮细胞有无影响及影响方式。方法用2%的毛果芸香碱给兔滴眼1个月和3个月后，取下兔眼球迅速固定，分离角膜内皮组织，用蛋白印迹（Western-blot）法检测兔眼角膜内皮细胞中抗凋亡基因bcl-2蛋白及凋亡基因bax蛋白的表达，流式细胞仪检测角膜内皮细胞的凋亡率，透射电镜下观察各组角膜内皮细胞的超微结构变化。结果应用毛果芸香碱1个月及3个月后，均呈现兔眼角膜内皮细胞凋亡基因bax蛋白表达增多，抗凋亡基因bcl-2蛋白表达减少，3个月组兔眼角膜内皮细胞凋亡率高于对照组（P＜0.05），透射电镜下观察可见，1个月及3个月组细胞内空泡形成，线粒体肿胀，内质网扩张，细胞核固缩，染色质固缩周边化等典型凋亡改变。结论长期应用2%的毛果芸香碱可以诱导兔眼角膜内皮细胞发生凋亡。

关键词: bcl-2蛋白 , 毛果芸香碱, bax蛋白, 角膜内皮, 细胞凋亡

Abstract: Objective To investigate the effects and mechanisms of pilocarpine on corneal endothelium in rabbits. Methods20 rabbits in the experiment group were subjected to 2% pilocarpine 3 times a day for 1 month or 3 months. The expressions of bcl-2 and bax protein were determined by Westernblot, the apoptosis rate of rabbit corneal endothelium cells was determined by flow cytometry, and the morphological changes of the corneal endothelium cells were determined by transmission electron microscopy. ResultsThe expression of bax protein in the experiment group was higher than that in the control group, however the expression of bcl-2 protein was lower than that in the control group. The apoptosis rate of the rabbit corneal endothelium cells using pilocarpine for 3 months was significantly higher than that of the control group (P＜0.05). Also the transmission electronic microscopy revealed there were morphological characteristics of apoptosis including swelling of organelles, increasing of vacuoles in the cytoplasm, compaction and margination of nuclear chromatin in the groups after using pilocarpine for 1 month and 3 months. Conclusion Topical use of 2% pilocarpine can induce the apoptosis of corneal endothelium cells in rabbits.

Key words: Apoptosis, Corneal endothelium, Pilocarpine, bax protein, bcl-2 protein

中图分类号:

R775.9

陶远,王红,乔智. 毛果芸香碱滴眼对兔眼角膜内皮细胞凋亡作用的影响[J]. 山东大学学报(医学版), 2007, 45(12): 1271-1274.

TAO Yuan,WANG Hong,QIAO Zhi. Effect of pilocarpine on apoptosis of corneal endothelium in rabbits[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(12): 1271-1274.

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