Abstract: Objective： To investigate the antivirus spectra of singlet compound named JN219 extracted from certain strains of fungi. Methods： Fifteen different strains of viruses， including herpesviridae virus（HCMV, HSV-1, HSV-2）, adenoviridae virus（Ad3）, paramyxoviridae virus（RSV）, reoviridae virus(RV SA11), orthomyxoviridae（influenza A, influenza B）, picornaviridae（COX B1-6）and rhabdoviridae（VSV）were used for in-vitro antivirus experiments, and the antiviral activity and the cytotoxicity of JN219 were evaluated by the cytopathic effect(CPE) method. Infected cells were dyed with 1％ neutral red, and the decolored and the absorptance A were read by an enzyme label meter. The cell survival rate, the median toxic concentration, the median inhibiting concentrations and therapeutic indexes were calculated by the Reed-Muench method. Antivirus stability experiments of JN219 were carried out at the same time. Results： Cytopathic effects caused by HCMV, VSV and RV SA11 were significantly inhibited. The median inhibiting concentrations (IC₅₀) were 1.42, 0.96 and 1.22μg/ml, respectively, and the therapeutic indexes (TI) were 35.26, 52.30 and 40.82, respectively. HSV-1， HSV-2， RSV， influenza A and influenza B were partially inhibited, however COX B1-6 and Ad3 were not inhibited. The cytotoxicity of the samples appeared only in the first dilution and the antiviral activity was unstable. Conclusion: JN219 has a wide antivirus spectra.

Key words: JN219, Fungus, Antivirus spectra