Journal of Shandong University (Health Sciences) ›› 2025, Vol. 63 ›› Issue (6): 1-10.doi: 10.6040/j.issn.1671-7554.0.2024.1116

• Preclinical Medicine •    

In vivo anti-aging effect of poria cocos three active ingredients on caenorhabditis elegans

YU Yunan1, WANG Ping2,3, DAI Junyi1, LIU Junfeng1,3,4, ZAN Junfeng1   

  1. 1. College of Pharmacy, Hubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei, China;
    2. Institute of Gerontology, Hubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei, China;
    3. Hubei Shizhen Laboratory, Wuhan 430065, Hubei, China;
    4. Key Laboratory of TCM Resources and Compounds of Ministry of Education, Hubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei, China
  • Published:2025-07-08

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Abstract: Objective To explore the anti-aging potential of Poria cocos by investigating the effects of its three active ingredients on the life-span and stress ability in Caenorhabditis elegans(C. elegans) and potential mechanisms. Methods Water-soluble polysaccharides, acidic polysaccharides, and triterpenes were extracted from Poria cocos using a solvent extraction method. The effects of these three active ingredients on the lifespan, stress resistance, reproduction ability, and locomotion of C. elegans, as well as their impact on the levels of ROS and lipofuscin, were evaluated. The expression of aging-related genes was determined using qRT-PCR. Results Compared with the control group, water-soluble polysaccharides at 500 μg/mL, acidic polysaccharides at 62.5 μg/mL, and triterpenes from Poria cocos at 500 μg/mL prolonged the lifespan of C. elegans by 12.72%, 15.07% and 12.55%, and enhanced the antioxidant stress resistance(P<0.05), respectively. Acidic polysaccharides and triterpenes increased their survival rates under heat stress and ultraviolet stress and reduced the levels of ROS in the C. elegans, with statistically significant differences(P<0.05). Meanwhile, these three active ingredients significantly reduced lipofuscin levels in C. elegans(P<0.05), and did not affect the egg-laying capacity and motility(P>0.05). The three active ingredients from Poria cocos upregulated the expression of daf-16 and ctl-2 mRNA, while downregulating the expression of daf-2 and age-1 mRNA, thereby extending the lifespan of C. elegans. Conclusion The three active ingredients of Poria cocos exhibited antioxidant and anti-aging effects in C. elegans. The mechanism may be related to regulating the expression of aging-related genes in the insulin/IGF-like signaling pathway.

Key words: Poria cocos active ingredients, Anti-aging, Water-soluble polysaccharides, Acidic polysaccharides, Triterpenes

CLC Number: 

  • R931.6
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