Abstract: To explore the expression of GTPCHⅠmRNA in aorta of diabetic and lipoic acid cured rats when the endothelial function was abnormal. Methods： Thirty five male Wistar rats were enrolled in this study; 10 of them were regarded as healthy control and 25 were given high fat diet, 8 weeks later they were administered STZ to the abdomen, finally only 21 became diabetic rats. The diabetic rats were divided into type 2 diabetes mellitus group(T2DM group, n=11) and alphalipoic acid cured group (LA group, n=10) who were given 50?mg/kg lipoic acid per day. After 16 weeks some indexes of three groups were measured, and EDVR in the isolated aortic rings were measured, and GTPCHⅠmRNA was measured by RTPCR in all rats′ aortas. Results： EDVR decreased obviously in both T2DM and LA group, that was better in LA group than in T2DM (P＜0.05). Compared with NC group, GTPCHⅠmRNA content in T2DM group decreased by 70.28%, and in LA group by 26.88%, the difference was significant(P＜0.05). Conclusions： The expression of GTPCHⅠmRNA in rats′ aorta decreases in type 2 diabetes mellitus with the attenuate of EDVR. Alphalipoic acid makes EDVR better in type 2 diabetes mellitus, which may correlate with the change of GTPCHⅠmRNA′s expression.

Key words: Diabetes mellitu?non insulidependen? GTPCH ｉ Lipoic aci? Endotheliu? vascula? Rat? Wista