Abstract: To clone the gene of human interleukin26（hIL26）and to construct its eukaryotic expression vector. Methods: Total RNA was extracted from the human peripheral blood monocytes，and reverse transcriptasepolymerase chain reaction（RTPCR）was performed. The PCR product was inserted into cloning vector pMD18T to construct the recombinant plasmid of hIL26pMD18T/hIL26. Recombinants were transformed into E.coliDH5α and screened with colony PCR and restriction analysis. The gene of hIL26 cloned was sequenced. The hIL26 gene fragment obtained from pMD18T/hIL26 was digested with SalⅠ and EcoRⅠ, and then inserted into pIRES2EGFP that was cut with XhoⅠ and EcoRⅠ. Recombinants were identified by colony PCR and restriction analysis. The recombinant expression plasmid pIRES2EGFPhIL26 was

Key words: Interleukin26, Gene cloning, COS7 cells, Eukaryotic expression vector