人外周血内皮前体细胞的分离、培养及流式细胞仪动态检测

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES)

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QIN Hongwei1,2, XIAO Dongjie1,　LIU Jie3,　HU An-la1,　MA Xiao-li1,　WANG Yun-shan1,　ZHANG Hong-wei4

1. Center of Medical Laboratory Diagnosis, Jinan Central Hospital, Clinical Medical College of Shandong University,Jinan 250013, Shandong, China; 2. Department of Biology, Jining Teachers′ College, Qufu 273155,Shandong, China

Received:2006-09-29 Revised:1900-01-01 Online:2006-12-24 Published:2006-12-24
Contact: QIN Hong-wei

Abstract

Abstract: To establish the method of isolation of endothelial progenitor cells（EPCs）from human peripheral blood and identify the markers of EPCs by FACS to provide basis for angiogenesis therapy. Methods: Total mononuclear cells (MNC) were isolated from peripheral blood by Ficoll density gradient centrifugation, Results：Adherent cells doublepositive for DilACLDL and FITCUEA1 could be observed under fluorescent and confocal laser microscope at 4 days after culture.KDR was (2.50±1.47)%,(5.72±1.66)% and (36.24±3.24)%, respectively at day 4 of culture. At days 5, 6 and 7, CD34+ cells were (8.45±3.97)%, (14.13±2.79)% and (21.14±2.91)% and CD31+ cells were (22.52±3.86)%， (42.76±3.67)% and (54.67±3.44)%, respectively. Conclusion：EPCs exist in the peripheral blood and can be differentiated into mature endothelial cells（ECs）. FACS can be used to efficiently identify EPCs for angiogenesis study.

Key words: Endothelial progenitor cells, Cell culture, Surface markers

QIN Hongwei,XIAO Dongjie,LIU Jie,HU An-la,MA Xiao-li,WANG Yun-shan,ZHANG Hong-wei. [J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(12): 1197-1201.

References

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