Abstract:

ObjectiveTo evaluate the impact of Aiolos gene over-expression on the cell cycle, apoptosis, chemotherapy sensitivity of leukemia cell lines and the potential mechanism. Methods   The Jurkat cells were divided into the Aiolos overexpression group, GFP transfected group and non-transfection group. Aiolos-overexpressed Jurkat cells were constructed by lentiviral transduction. Then the cell cycle, apoptosis, chemotherapy sensitization and expression of related genes were detected. Results   Expression of Aiolos was up-regulated 4 days after transfection. Over-expression of Aiolos promoted cell apoptosis and arrested cell cycle into S phase. In addition, over-expression of Aiolos led to up-regulation of p21, p27 (P<0.05) and down-regulation of cyclin D3, Skp2 and Bcl-2 (P<0.05). No changes were detected on Bax. However, there was an increase in the Bax to Bcl-2 ratio. Furthermore, the cell survival rates exposed to 40μg/mL etoposide were reduced in the Aiolos overexpression group compared with those in the control groups at 36h and 48h (P<0.05). Conclusion    Recombinant lentiviral vectors of Aiolos gene overexpression promote cell apoptosis, arrest cell cycle and enhance etoposide cytotoxity in Jurkat cells.

Key words: Aiolos; Acute lymphocytic leukemia; Gene transfection; Jurkat cell;  Chemosensitivity