Abstract:

Objective   To screen the differentially expressed microRNAs in the CD138+ cells of multiple myeloma (MM) patients with deletion of chromosome 13 and explore the biological functions of them. Methods   3 cases with chromosome 13 deletion and another 3 with normal karyotype from 29 previously untreated multiple myeloma patients were screened by fluorescence in situ hybridization (FISH). The miRNA microarray was used to detect the differentially expressed miRNAs, and the real-time PCR was used to verify the results. The potential target genes of the detected differentially expressed miRNAs were predicted by online software. Then the regulatory network of miRNAs and target genes was established. Results   Five differentially expressed miRNAs were detected in the CD138+ cells of MM patients with deletion of chromosome 13, of which four were down-regulated and one was up-regulated obviously. Bioinformatic analysis showed that SMAD3 was in the key node of the miRNA-target gene regulatory network. Conclusion   The multiple myeloma patients with chromosome 13 deletion have their specifically expressed miRNAs. The gene of SMAD3 may play an important role in the pathogenesis of this type of disease.

Key words: miRNA; Multiple myeloma; Microarray; Target gene；SMAD3