Abstract:

Objective   To investigate the relationship between Clostridium leptum (C.leptum) subgroup in the human gut, the periodontal disease and type 2 diabetes mellitus. Methods   Fresh fecal samples were obtained from twenty patients with chronic periodontitis (CP group), twenty patients with type 2 diabetes mellitus (DM group), twenty patients with chronic periodontitis complicated by type 2 diabetes ［(DM+CP) group］ and twenty healthy control subjects (HC group). Genus-specific primer of C.leptum subgroup was designed for 16S rRNA-targeted PCR. The standard curve of real-time quantitative polymerase chain reaction (RTQPCR) was generated from a grade-dilution of plasmid DNA which was cloned from PCR products. Bacterial genome DNA extracted from fecal samples was quantified by RTQ-PCR to analyze C.leptum subgroup amounts. Results   Logarithm for 16S rRNA gene copies of C.leptum subgroup per gram feces in HC group, CP group, DM group and (DM+CP) group were (7.09±0.17), (6.31±0.58), (5.79±0.52) and (5.41±0.29), respectively. Compared with HC group, the logarithm of the other three groups were dramatically decreased (P＜0.05). Conclusion   C.leptum subgroup may be associated with the development and progression of chronic periodontitis and type 2 diabetes mellitus.

Key words: Chronic periodontitis; Type 2 diabetes mellitus; Clostridium leptum subgroup； Real-time quantitative polymerase chain reaction