JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2012, Vol. 50 ›› Issue (12): 94-.

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The effect of methyltransferase inhibitor on the expression of
XAF1 mRNA in prostate cancer

GUO Xiao-yu1, JIANG Yan2, XING Zhao-quan3, GUO Zhao-xin3, FANG Zhi-qing3, LIU Zhao-xu1,3   

  1. 1. School of Nursing, Shandong University, Jinan 250012, China;
    2. The People’s Hospital of Rushan, Rushan  264500, Shandong, China;
    3. Department of Urology, Qilu Hospital of Shandong University, Jinan  250012, China
  • Received:2012-07-31 Online:2012-12-10 Published:2012-12-25

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Abstract:

Objective   To investigate the expression of XAF1 mRNA and the effect of DNA methylation inhibitor on its expression in prostate cancer.  Methods   Culture prostate cancer cell lines LNCaP, DU145, PC3 cells and renal cell carcinoma-derived A498 cells.Extract normal peripheral blood mononuclear cells (PBMCs). A498 cells and PBMCs were used as positive controls. Collect 10 clinical cases of prostate cancer and neighboring prostate tissue as control. RT-PCR was performed in order to detect the XAF1 mRNA experssion in LNCaP, DU145, PC3, A498 and PBMCs. Methylation-specific PCR(MSP) was used to detect the XAF1 experssion in LNCaP and DU145 cells before and after they were treated with DNA methylation inhibitor (5′-aza).   Results   LNCaP and DU145 prostate cancer cell lines expressed a shorter form of XAF1 transcripts than the A498 cells, whereas PC3 cells exhibited a complete silence of the XAF1 gene.Yet,after being treated with 5′-aza, LNCaP and DU145 expressed the full length of XAF1 mRNA. Conclusion   Low expression of XAF1 mRNA is found in LNCaP and DU14 cells. No expression is found in PC3 cells.DNA methylation inhibitor can induce the full-length of XAF1 mRNA expression in prostate cancer cells.

Key words: Suppressor gene； DNA methylation； DNA methylation inhibitor； Prostate neoplasm； Alternative splicing

CLC Number: 

  • R737.25
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