Abstract:

Objective   To investigate the influence of mild hypothermia on the expression of nuclear factor-kappa B(NF-κB) in cerebral microvascular endothelial cells injured by hydrogen peroxide. Methods   The cells of rat cerebral microvascular endothelial were separated and cultured, and the cell model of inflammatory response after cerebral hemorrhage was established by the method of hydrogen peroxide injury. Cells were divided into the experiment group(ICH) and the control group(C). Each group was divided into the normothermia  group(N ) and the mild hypothermia group(H). The expression of NF-κB was detected by western blotting after hydrogen peroxide intervention. Results   The expression of NF-κB obviously increased in the experiment group compared with the control group(P<0.01). While the level of  NF-κB was higher in the normothermia group than that in the mild hypothermia group(P<0.01).  Conclusion   Mild hypothermia may protect perihematoma tissues through relieving the inflammation by inhibiting NF-κB over-expression.

Key words: Hypothermia; Cerebral hemorrhage; NF-kappa B; Endothelium, vascular; Inflammation