Abstract:

Objective   To explore the effects of resveratrol on expressions of interleukin-1(IL-1β), interleukin-6(IL-6), monocyte chemotactic protein-1(MCP-1) and suppressor of cytokine signaling1/3(SOCS1/3) in cultured human peripheral blood mononuclear cells (PBMCs). Methods    Cultured PBMCs were treated with different concentrations of resveratrol (0, 3.1, 6.25, 12.5, 25μg/mL ). Expression of IL-1β, IL-6 and MCP-1 at both mRNA and protein levels, SOCS1/3 at mRNA level were determined using real-time quantitative PCR and ELISA. Results   Compared with the control group（0μg/mL）, expression of IL-1β, IL-6 and MCP-1 were significantly suppressed by resveratrol, while expression of SOCS1/3 were increased (P<0.05). The levels of IL-1β and IL-6 proteins expression significantly decreased at the concentration of 25μg/mL(P<0.05), and the level of MCP-1 protein expression decreased in groups from 6.25μg/mL to 25μg/mL(P<0.05). IL-β, IL-6 and MCP-1 mRNA expression were significantly suppressed while SOCS1/3 mRNA expression was highly induced by resveratrol at all groups(P<0.05). Conclusion   Resveratrol can inhibit expression of the pro-inflammatory cytokines IL-1β, IL-6, MCP-1 and induce expression of SOCS1/3 in PBMCs at a dose dependent manner, which further indicates its anti-inflammatory function in pre-clinical studies.

Key words:  Peripheral blood mononuclear cells; Resveratrol; Cytokine;  Suppressor of cytokine signaling