JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2011, Vol. 49 ›› Issue (11): 44-.
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LI Lu1, LIU Ling2, LIU Xiao-li2, LIU Jun-li2, ZHAO Jing-jie2,, SHANG Wei1
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Abstract:
Objective To construct a subtracted cDNA library of differentially expressed genes in the hippocampus of rats with temporal lobe epilepsy, and to screen for differentially expressed genes related to temporal lobe epilepsy. Methods Suppression subtractive hybridization (SSH) was used for isolating cDNA fragments of differentially expressed genes in the hippocampus of temporal lobe epilepti rats, and normal hippocampus tissues were controls. The products of SSH were directly inserted into the T/A cloning vector to set up the subtractive library which was amplified through E.coli transformation. One hundred positive bacterium clones were randomly picked and screened the colony polymerase chain reaction (PCR) method. Results Fourteen differentially expressed genes were obtained from the hippocampus of temporal lobe epileptic rats by sequencing and using Basic Local Alignment Search Tool (BLAST), and they were highly homologous with the known gene fragments in rats. Conclusion SSH is an effective method for screening for differentially expressed genes. Many differentially expressed genes exist in the hippocampus of rats with temporal lobe epilepsy. The results of this study provide an important clue for further exploring the molecular mechanism of temporal lobe epilepsy.
Key words: Suppression subtractive hybridization; Epilepsy, temporal lobe; Hippocampus; Gene expression
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LI Lu1, LIU Ling2, LIU Xiao-li2, LIU Jun-li2, ZHAO Jing-jie2, SHANG Wei1. Application of suppression subtractive hybridization to screen for temporal lobe epilepsy-related genes[J].JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(11): 44-.
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