Abstract:

Objective   To detect the effect of lidocaine on expression of high mobility group protein box 1(HMGB1) in kidneys of septic rats and investigate its protective mechanism against acute kidney injury. Methods   Wistar rats, subjected to cecal ligation and puncture (CLP), were treated with normal saline or lidocaine of 5, 10 and 20mg/kg at 0 and 1, 2 hour after CLP.  Twenty-four hours after CLP, the level of HMGB1 mRNA and activation of NF-κB p65 in kidneys were assessed, and alteration of histology and concentration of plasma creatinine were determined.  Results   ① Compared with the sham-operated group, the level of HMGB1mRNA in kidneys was significantly increased in rats subjected to CLP, which was suppressed by lidocaine in a dose-dependent manner(P<0.05).  ② Concentrations of plasma creatinine in groups treated with lidocaine of 5, 10 and 20mg/kg ［(44.80±3.70), (34.80±4.44) and (27.40±2.30)μmol/L］ were significantly decreased compared with the saline-treated group ［(51.00±5.0)μmol/L, P<0.05］. ③ Infiltration of inflammatory cells and histological damages induced by CLP were mitigated by lidocaine. ④ Activation of NF-κB p65 in kidneys was also inhibited by lidocaine.  Conclusions   The protective effect of lidocaine on CLP-induced acute kidney injury may be result from its inhibition of expression of HMGB1 in tissues.

Key words: Lidocaine; Sepsis; High mobility protein box 1; Acute kidney injury