JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2011, Vol. 49 ›› Issue (9): 102-.

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MB-WCX coupled with SELDI-TOF-MS: application to the diagnosis of gastric cancer

LIU Bo-xue1, CHENG Huan-huan2, DONG Zhao-gang3, WANG Hua-yang2, YIN Zuo-hua3, SONG Qing-xu2   

  1. 1. Department of Medical Engineering, Qilu Hospital of Shandong University, Jinan 250012, China;
    2. Institute of Basic Medical Sciences, Qilu Hospital of Shandong University, Jinan 250012, China;
    3. Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, China
  • Received:2011-02-20 Online:2011-09-10 Published:2011-09-10

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Abstract:

Objective   To explore the validity of MB-WCX coupled with SELDI-TOF-MS on the diagnosis of gastric cancer and its precancerosis. Methods   MB-WCX coupled with SELDI-TOF-MS was used to analyze the proteomic profiles of sera from 26 patients with gastric cancer, 20 patients with gastric ulcer, 15 patients with atrophic gastritis, and 30 healthy individuals. The differentially expressed proteins were examined by comparing to the Swiss protein database, according to their molecular weights and isoelectric points. Results   Three proteins at 2741.2, 7963.6 and 15904.3m/z were up-regulated in patients with gastric cancer or precancerosis, relative to healthy individuals. A fourth one at 13732.6m/z was down-regulated in patients with gastric cancer, but showed little difference between precancerosis and healthy conditions. These four proteins, in combination, can be used to detect gastric cancer, with a sensitivity of 92.3%, and a specificity of 96.7%. Conclusion   MB-WCX coupled with SELDI-TOF-MS is an effective means to screen for biomarkers. The four low molecular weight serum proteins can be hopeful biomarkers for gastric cancer.

Key words: Surface-enhanced laser desorption ionization time-of-flight mass spectrometry; weak cation exchange magnetic beads; Gastric cancer; Precancerous conditions; Diagnosis

CLC Number: 

  • R735.2
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