Abstract:

Objective   To investigate the effects of BRMS1 on  tumorigenicity and inhibition for the distant metastasis of implanted human rectal carcinoma in  nude mice. Methods   The experimental transplanted and spontaneous transplanted models were constructed in nude mice by transferring BRMS1 gene into human rectal cancer cell line LOVO. Growth of nude mice and distant metastasis of cancer cells in transfected group(A), non-carrier group(B) and non-transfected group(c) were observed. Results    The spontaneous transplanted model : there were no significant differences on the average diameter of inoculated tumor and the body mass among groups A1, B1 and C1. The experimental transplanted model : the pulmonary metastasis rates were 100% in groups B2 and C2, but diffuse infiltration was the main form in pulmonary tissues; the pathology degrees of pulmonary and hepatic metastasis of group A2 were slightly decreased compared with groups B2 and C2; the pulmonary metastasis degree of group A2 was mainly grade Ⅱ, and mainly grades Ⅱ and Ⅲ in groups B2 and C2; liver metastasis was not detected in group A2;the pathology degrees of liver metastasis in groups B2 and C2 were mainly grades Ⅰ and Ⅱ. Conclusion   BRMS1 can inhibit the distant metastasis of rectal carcinoma LOVO cells, but has no effect on the tumor growth, which suggests that the BRMS1 gene could be a new target for the inhibition on the colorectal cancer distant metastasis.

Key words: reast cancer metastasis suppressor 1; Rectal neoplasms; Gene therapy; Distant metastasis；Metastasis suppressor genes