Abstract:

Objective    To explore expressions of programmed cell death 5(PDCD5) and P53 in skin lesions of patients with granuloma fungoides(mycosis fungoides, MF) at different stages.  Methods    Specimens were divided into 3 groups, the patch stage MF group(n=19), the plaque/tumor stage MF group(n=5) and the control group(n=10).   Expressions of PDCD5 and P53 were detected in epidermal keratinocytes and dermal lymphocytes of all the specimens by immunohistochemistry.  Results     ① Overexpression of the PDCD5 protein was observed in epidermal keratinocytes and dermal lymphocytes of the control group and the patch stage MF group, and there was no significant difference in the number of PDCD5+ cells between the two groups(P>0.05).  The PDCD5+ cells in the epidermis of the plaque/tumor stage MF group were less than those in the control group and the patch stage MF group(P<0.05).  ②  Expression of P53 was not detected in epidermal keratinocytes of the control group, the P53 protein was positively detected in 36.84%(7/19) of  patients with patch stage MF and in 100%(5/5) of patients with plaque/tumor stage MF.  And there were statistically significant differences between any two groups(P<0.05).  There was no expression of P53 in the dermis of the control group and the patch stage MF, and P53 was positively detected in 20%（1/5） of patients with plaque/tumor stage MF.  But there was no significant difference between any two groups(P>0.05).  ③ There was no correlation between expressions of PDCD5 and P53 in the epidermis and dermis of MF at different stages(P>0.05).  Conclusion    Expression of PDCD5 in epidermal keratinocytes and dermal lymphocytes gradually decreased with progression of MF, while expression of P53 in epidermal keratinocytes gradually increased during that process, which suggested that PDCD5 and P53 may play an important role in tumorigenesis of MF.

Key words: Programmed cell death 5; Granuloma fungoides; Protein, P53; Keratinocytes