JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2010, Vol. 48 ›› Issue (11): 20-.

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Role of Tribble 3 on the production of collagen type Ⅰby cardiac fibroblasts induced by high glucose

TANG Meng-xiong1,2,  L Rui-juan1,2, ZHONG Ming2,3, ZHANG Yun2,3, ZHANG Wei2,3, CHEN Yu-guo1,2   

  1. 1.Department of Emergency, Qilu Hospital of Shandong University, Jinan 250012, China;
    2. Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education
    and Public Health, Jinan 250012, China; 
    3. Department of Cardiology, Qilu Hospital of Shandong University, Jinan 250012, China
  • Received:2010-09-10 Online:2010-11-16 Published:2010-11-16

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Abstract:

Objective    To investigate the role of Tribble 3(TRIB3) on the production of collagen type Ⅰby cardiac fibroblasts induced by high glucose. Methods    Rat cardiac fibroblasts were cultured in vitro with different concentrations of glucose. mRNA expressions of collagen type Ⅰand TRIB3 were measured by real time-PCR, and protein level of collagen type Ⅰwas determined by enzyme-linked immunosorbent assay. siRNA targeting TRIB3 was transfected into cardiac fibroblasts, and then the production of pro-collagen type Ⅰby cardiac fibroblasts induced by high glucose was detected. Results     Both mRNA and protein expressions of collagen type Ⅰwere increased after being induced by high glucose（P＜0.05）, and mRNA expression of TRIB3 was also increased（P＜0.05）. After inhibiting expression of TRIB3 by siRNA, the increased mRNA and protein levels of collagen type Ⅰinduced by high glucose were reversed（P＜0.05）. Conclusion     TRIB3 is involved in the production of collagen type Ⅰby cardiac fibroblast induced by high glucose.

Key words: High glucose; Cardiac fibroblasts; TRIB3; Collagen type Ⅰ; Rat, Wistar

CLC Number: 

  • R542.2
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