Abstract:

Objective   To explore the feasibility of Xinmailong injection inducing rat bone marrow mesenchymal stem cells (BMSCs) to differentiate into neuronlike cells. Methods   The rat BMSCs were separated and purified with the adherent culture method. Following passages, the cell surface markers CD29, CD90, CD34 and CD45 were measured by flow cytometry. The expressions of Nestin, Neuron specific enolase (NSE), Microtubule associated protein2(MAP2) and Glial fibrillary acidic protein (GFAP) were detected by immunofluorescence assay. Results   Flow cytometry showed positive expression of CD29 (99.06%) and CD90 (99.61%) and negative expression of CD34 (2.03%) and CD45 (2.00%). At 4h, 12h after induction, the positive rates of Nestin were (81.0±1.6)%, (22.5±1.9)%, while at 36h after induction, Nestin was negative. At 4h after induction, NSE and MAP2 were negative. At 12h, 36h after induction, the positive rates of NSE were (73.5±2.2)%, (94.3±1.8)% and those of MAP2 were (80.0±2.2)%, (96.4±2.8)%. At 4h, 12h, 36h after induction, GFAP was nagative. Conclusion   Xinmailong injection can rapidly and efficiently induce rat BMSCs to differentiate into neuron like cells.

Key words: Stem cells; Cell differentiation; Neurons; Rats; Periplaneta