Abstract:

Objectiv  eTo establish insulin resistance model by intraperitoneal injection of streptozotocin (STZ) and investigate the relationship between high mortality and altered expression of Klotho in insulin resistance rats. Methods  Selecting 20 from 80 Wistar rats born on the day for the normal control group (CN group, n=20), Streptozotocin was injected into abdominal cavity to induce insulin resistance in both insulin resistance group and treated group in remaining 60 Wistar rats . Selecting 40 to establish insulin resistance group (IR group, n=20), Tetramethylpyrazine and aminoguanidine treatment group (TMP + AG group, n=20). Plasma glucose, glycosylated serum protein were detected by taking blood at 8 weeks, 24 weeks,32 weeks respectively after model. After model of 32 weeks, Klotho mRNA was measured by RT-PCR, The expression of Klotho protein in the kidney was detected by immunohistochemistry and Western-blot. Result  sThe expression of Klotho mRNA and protein of kidney increased significantly  in TMP+AG group (P<0.05), the mortality rate dropped markedly in comparison with those in insulin resistance group(P<0.05). The expression of Klotho mRNA and protein of kidney increased significantly in insulin resistance group and the mortality rate increased in comparison with those in control group( P<0.05). Conclusion  The level of Klotho expression was negatively correlated with the mortality rate in insulin resistance rat.

Key words: Klotho; Mortality; Insulin resistance; Aminoguanidine; Tetramethylpyrazine; Rats, Wistar