Abstract:

Objective   To investigate the relationship between multiple drug resistance 1(MDR1) polymorphism and prednisone sensitivity in ulcerative colitis (UC) patients. Methods   86 UC patients were divided into three groups according to their history: 30 newly diagnosed UC patients, 30 UC patients who were previously sensitive to the prednisone treatment and 26 UC patients on whom the prednisone treatment had no effect. DNA samples were obtained from 86 UC patients. Genotypes of the MDR1C3435T and G2677T/A loci were determined by polymerase chain reaction of the sequence specific primer (PCR-SSP). Results   The MDR1C3435T locus consisted of three genotypes: CC,CT and TT. There were significant differences between the newly diagnosed UC patients and prednisone resistant patients in the MDR1C3435T locus. The same results occurred in prednisone resistance patients and prednisone sensitive patients. However there was no significant difference in C and T allele frequencies among the three groups in the MDR1C3435T locus. There was no significant difference of the genotypes and allele frequencies of the G2677T/A locus among the three groups. Conclusion   There is a relationship between the MDR1C3435T polymorphism and prednisone resistance UC patients in Chinese Han nationality. The MDR1C3435T gene mutation in UC patients is prone to the prednisone resistance. The result can not prove there is a correlation between UC in Chinese Han nationality and MDR1 G2677T/A polymorphism.

Key words: Ulcerative colitis； MDR1C3435T and G2677T/A gene polymorphism； Prednisone sensitivity； P-glycoprotein