Abstract: To explore the relationship between single nuclear polymorphism (SNP) of 1188A/C in the interleukin 12B gene and susceptibility of chronic obstructive pulmonary disease (COPD) in the Chinese Han population from Shandong Province. MethodsSNP 1188A/C of the IL12B gene was detected by polymerase chain reaction (PCR) based on the restriction fragment length polymorphism (RFLP) technique in 324 COPD subjects and 389 healthy controls. ResultsThe frequencies of genotypes AA, AC, and CC at polymorphism sites 1188A/C were 0.333?3, 0.478?4, and 0.188?3 respectively in the COPD group, while they were 0.357?3, 0.475?6, and 0.167?1 in the control group. Also, the A type allele frequencies were 0.572?5 and 0.595?1 in patients and controls, and the C type allele frequency was 0.427?5 and 0.404?9 in COPD patients and healthy controls. There were no significant differences in genotype frequencies (P value=0.689) or allele frequencies between the two groups (P=0.389). ConclusionNo significant association was found between IL12B 1188A/C polymorphism and COPD susceptibility in the Chinese Han population from Shandong Province.

Key words: Interleukin12, Pulmonary disease,chronic obstructive, Genetic predisposition to disease