苦参碱对人结肠癌细胞株HT-29体外侵袭能力的抑制作用

山东大学学报(医学版)

苦参碱对人结肠癌细胞株HT-29体外侵袭能力的抑制作用

丁印鲁¹,唐思锋¹，王敬源¹,付勤烨¹，张建良¹，李占元²，李兆亭²

山东大学 1. 第二医院普通外科，山东济南 250033； 2. 齐鲁医院普通外科, 山东济南　250012

收稿日期:2007-01-15 修回日期:1900-01-01 出版日期:2007-09-24 发布日期:2007-09-24
通讯作者: 丁印鲁

Inhibitory effect and mechanism of matrine on the invasive ability of the colon cancer cell line HT-29 in vitro

DING Yin-lu¹,TANG Si-feng¹，WANG Jing-yuan¹,FU Qin-ye¹，ZHANG Jian-liang¹，LI Zhan-yuan²，LI Zhao-ting²

Department of General Surgery, １. Second Hospital of Shandong University， Jinan 250033, Shandong, China；2. Qilu Hospital of Shandong University, Jinan 250012, Shandong, China

Received:2007-01-15 Revised:1900-01-01 Online:2007-09-24 Published:2007-09-24
Contact: DING Yin-lu

摘要/Abstract

摘要： 目的：探讨苦参碱对人结肠癌细胞株HT-29体外侵袭能力及乙酰肝素酶(heparanase, HPSE)基因表达的影响。方法：HT-29细胞体外培养,以0.125、0.25、0.5mg/ml不同浓度苦参碱进行预处理48h后,采用半定量RT-PCR、Western-blot检测各组培养细胞HPSE mRNA及HPSE蛋白表达的变化,采用细胞黏附实验检测细胞黏附能力的改变,Transwell小室检测侵袭及趋化能力的变化。结果：与对照组比较,各浓度苦参碱处理组HT-29细胞HPSE mRNA及HPSE蛋白的表达和细胞黏附侵袭能力均受到显著的抑制(P＜0.01),其抑制效应与药物的浓度呈正相关(组间比较P＜0.01)。结论：苦参碱能够显著抑制细胞的体外侵袭能力，其机制可能与下调HT-29细胞HPSE基因表达有关。

Abstract: Objective： To investigate the effect of matrine on the invasiveness and expression of heparanas-mRNA in the human colon cancer cell line HT-29. Methods： HT-29 cells were subjected to matrine of different concentrations. Fortyeight hours later, the expression of heparanase-mRNA was determined by semi-quantitative RTPCR, and the level of HPSE protein by western blot. The effect of matrine on HT29 cells was tested by cell Matrigel adhesion assay. The chemotactic migration and the invasive ability of HT-29 cells were determined by Matrigel invasion assay. Results： The expression of hepanase-mRNA and hepanase protein, adhesion, chemotactic migrations and invasiveness of matrine treated HT-29 cells significantly decreased compared with those of the controls(P＜0.01). The inhibitory effect was dose-dependent(P＜0.01). Conclusion： By down-regulating the expression of heparanase-mRNA and hepanase protein, matrine has a significant inhibitory effect on the adhesion and invasiveness of the human colon cancer cell line HT-29 in a dose-dependent manner within a certain range.

Key words: Colon neoplasms, Heparanase, Matrine, Invasiveness

中图分类号:

R730.52

丁印鲁,唐思锋,王敬源,付勤烨,张建良,李占元,李兆亭 . 苦参碱对人结肠癌细胞株HT-29体外侵袭能力的抑制作用[J]. 山东大学学报(医学版), 2007, 45(9): 891-894.

DING Yin-lu,TANG Si-feng,WANG Jing-yuan,FU Qin-ye,ZHANG Jian-liang,LI Zhan-yuan,LI Zhao-ting. Inhibitory effect and mechanism of matrine on the invasive ability of the colon cancer cell line HT-29 in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(9): 891-894.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed