Aβ1-42对大鼠基底前脑神经元KATP通道各亚基蛋白表达的影响

山东大学学报(医学版)

Aβ_1-42对大鼠基底前脑神经元KATP通道各亚基蛋白表达的影响

付庆喜¹,马国诏¹,高建新²,毕爱玲²,刘克敬²,张镛¹,郑敏¹,刘振芳³

1. 山东大学山东省立医院神经内科，济南 250021; 2. 山东大学医学院生理研究所，济南 250012; 3. 青岛大学医学院附属医院东区急诊内科，山东青岛 266101

收稿日期:2007-07-03 修回日期:1900-01-01 出版日期:2007-11-24 发布日期:2007-11-24
通讯作者: 马国诏

Effects of Aβ_1-42on the subunits of KATP expression of cultured primitive basal forebrain neurons in rats

FU Qing-xi¹,MA Guo-zhao¹,GAO Jian-xin²,BI Ai-ling²,LIU Ke-jing²,ZHANG Yong¹,ZHENG Min¹,LIU Zhen-fang³

1. Department of Neurology, Shandong Provincial Hospital; 2. Department of Physiology, School of Medicine, Shandong University; 3. Department of Emergency, Affiliated Hospital of School of Medicine

Received:2007-07-03 Revised:1900-01-01 Online:2007-11-24 Published:2007-11-24
Contact: MA Guo-zhao

摘要/Abstract

摘要： 目的研究β淀粉样蛋白（Aβ_1-42）对原代培养基底前脑胆碱能神经元ATP敏感性钾通道（KATP）各亚基蛋白表达的影响，探讨阿尔茨海默病发病的细胞毒性分子机制。方法运用细胞原代培养的方法培养大鼠基底前脑胆碱能神经元并进行鉴定, 用2μmmol/L的Aβ_1-42对原代培养细胞进行干预, 免疫荧光双染及免疫印记观察干预后不同时间(分别为0, 24, 72h)细胞KATP通道各亚基Kir6.1、Kir6.2和SUR1、SUR2蛋白表达水平的变化。结果与正常对照组比较，Aβ_1-42作用胆碱能神经元24h后, KATP通道亚基Kir6.1及SUR2蛋白表达显著增多(P<0.05)，而亚基Kir6.2及SUR1蛋白表达无明显变化。但Aβ_1-42作用时间达72h后, KATP通道各个亚基蛋白表达均显著升高(P<0.05)。结论 Aβ_1-422作用胆碱能神经元不同的时间段（24h和72h），细胞KATP通道各亚基蛋白表达有不同程度的增加，且增加速度不一致。可能由此改变KATP通道的结构和功能，从而影响Aβ_1-42的神经细胞毒性作用。

关键词: ATP敏感性钾通道 , 基底前脑, 胆碱能神经元, β淀粉样蛋白

Abstract: ObjectiveTo investigate the effects of Beta-amyloid peptides (Aβ_1-42) on the subunits of KATP expression of cultured primitive basal forebrain cholinergic neurons in rats. Methods Primitive rat basal forebrain neurons were cultured and evaluated. The subunits of KATP: Kir6.1, Kir6.2, SUR1 and SUR2 expression were determined by double immunofluorescence and immunoblotting when the neurons were exposed to Aβ_1-42 (2μmmol/L) for different times.Results After being exposed to Aβ_1-42 for 24 hours, the expression of subunits Kir6.1 and SUR2 in the cultured neurons was significantly increased compared with the controls (P<0.05), while that of Kir6.2 and SUR1 had no significant difference from that of the controls. After being exposed to Aβ_1-42 for 72 hours, the expressions of the four subunits were all significantly increased compared with the controls (P<0.05). Conclusion Aβ_1-42 for different times (24h and 72h) induces different regulation of KATP subunit expressions in cultured primitive rat basal forebrain cholinergic neurons. Changes in composition of KATP may resist the toxicity of Aβ_1-42.

Key words: Beta-amyloid peptides, Basal forebain, Cholinergic neuron, ATP-sensitive potassium channel

中图分类号:

R749.16

付庆喜,马国诏,高建新,毕爱玲,刘克敬,张镛,郑敏,刘振芳 . Aβ_1-42对大鼠基底前脑神经元KATP通道各亚基蛋白表达的影响[J]. 山东大学学报(医学版), 2007, 45(11): 1162-1167.

FU Qing-xi,MA Guo-zhao,GAO Jian-xin,BI Ai-ling,LIU Ke-jing,ZHANG Yong,ZHENG Min,LIU Zhen-fang. Effects of Aβ_1-42on the subunits of KATP expression of cultured primitive basal forebrain neurons in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(11): 1162-1167.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed

[1]	李梅, 孟庆慧, 蔡巧英, 徐雁, 范晓婷. 乙酰葛根素对Aβ_25-35诱导BV-2小胶质细胞Caspase-3表达的影响[J]. 山东大学学报（医学版）, 2015, 53(10): 32-36.
[2]	李艳菊，谭淑慧，夏春凤，张镛，杜怡峰，马国诏. NF-κB对Aβ1-42诱导神经元KATP亚基Kir6.2/SUR1表达的影响[J]. 山东大学学报(医学版), 2013, 51(10): 5-9.
[3]	殷青青，刘雪平，董传芳，董雪丽，李艳菊, 罗鼎真，侯训尧. 糖基化终末产物对大鼠基底前脑胆碱能神经元的损伤作用[J]. 山东大学学报(医学版), 2012, 50(8): 20-.
[4]	孙锡波，韩玉香，韩涛，王雪，赵秀鹤，刘学伍，迟兆富. 二氮嗪对氯化锂-匹鲁卡品致痫大鼠海马神经元超微结构及自由基的影响[J]. 山东大学学报(医学版), 2011, 49(2): 19-23.
[5]	蒋亮亮1 ，付庆喜2 ，王瑞霞1 ，马国诏1 ，高建新3 ，刘克敬3 ，张镛1 . 二氮嗪、环孢菌素A拮抗Aβ142致胆碱能神经元凋亡的研究[J]. 山东大学学报(医学版), 2009, 47(03): 23-29.