月腺大戟水提物对小鼠的毒性作用

山东大学学报(医学版)

月腺大戟水提物对小鼠的毒性作用

居学海¹，崔　晞¹，陈鸣岳¹，杜　娟²，王淑娥¹，崔兰漪³

1. 山东大学公共卫生学院卫生检验学研究所，山东济南 250012；2. 山东大学齐鲁医院药剂科，山东济南 250012；3. 山东省金泰生物工程公司，山东　济南 250100

收稿日期:2005-12-08 修回日期:1900-01-01 出版日期:2007-01-24 发布日期:2007-01-24
通讯作者: 崔　晞

Toxicity of Euphorbia ebiacteolata Hayata water extract on mice

JU Xue-hai¹，CUI Xi¹，CHEN Ming-yue¹，DU Juan²，WANG Shu-e¹，CUI Lan-yi³

1. Department of Chemistry and Bacteria Detection, School of Public Health, Shandong University, Jinan 250012；2. Department of Pharmaceutical Preparation, Qilu Hospital of Shandong University；3. Jintai Group Co., LTD, Jinan 250100, Shandong, China

Received:2005-12-08 Revised:1900-01-01 Online:2007-01-24 Published:2007-01-24
Contact: CUI Xi

摘要/Abstract

摘要： 目的：探讨月腺大戟水提物的毒性作用。方法:以实验用一级标准昆明种近交系小鼠为实验对象,随机分为5组。阴性对照组，给予蒸馏水灌胃；阳性对照组，腹腔注射环磷酰胺50mg·kg^-1；低、中、高剂量组，分别给予77、144、288g·kg^-1月腺大戟水提物灌胃，观察微核率、精子畸变率以及高剂量组的肝、脾、肾、心、脑、肺病理切片。结果:高剂量(288g·kg^-1) 月腺大戟水提物对脾脏、肾脏和心脏具有毒性作用；中、高剂量组的微核率和精子畸变率与阴性对照组均有统计学差异(P＜0.01, P＜0.005)，低剂量组与阴性对照组无统计学差异(P＞0.05)。结论:月腺大戟水提物在较高剂量下有致突变和生殖毒性，但在低剂量下未发现明显毒性。

Abstract: Objective: To investigate the potential toxicity of Euphorbia ebiacteolata Hayata (EEH) water extract. Methods: The study was performed on inbred Kunming mice, using the traditional toxicological method based on observing ratios of micronucleus and sperm abnormity (RMS). The mice were randomly divided into 5 groups: a negative control with distilled water, a positive control with cytoxan(50mg·kg^-1), and low dose, medium, and high dose of EEH extract(77g·kg^-1, 144g·kg^-1 and 288g·kg^-1 respectively). Results: The results showed that the high and medium doses significantly increased RMS compared with the negative controls (P＜0.01, P＜0.005), but a similar result was not observed in the low dose group (P＞0.05). Conclusion: EEH can induce mutation and sperm abnormity in medium and high doses, whereas it cannot induce significant toxicity in low doses.

Key words: Euphorbia ebiacteolata Hayata, Mutation, Toxicity, Mice

中图分类号:

R284.2

居学海,崔　晞,陈鸣岳,杜　娟,王淑娥,崔兰漪 . 月腺大戟水提物对小鼠的毒性作用[J]. 山东大学学报(医学版), 2007, 45(1): 62-64.

JU Xue-hai,CUI Xi,CHEN Ming-yue,DU Juan,WANG Shu-e,CUI Lan-yi. Toxicity of Euphorbia ebiacteolata Hayata water extract on mice[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(1): 62-64.

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