PTTG、VEGF-C 基因过表达与非小细胞肺癌发生、发展的关系及其临床意义

PTTG、VEGF-C 基因过表达与非小细胞肺癌发生、发展的关系及其临床意义

Overexpressions of PTTG and VEGFC in nonsmall cell lung cancer and their clinical significances

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山东大学学报(医学版)

郭旭峰¹,陈蔚文²,胡国强¹,尹秋伟¹,赵健¹

山东大学 1. 齐鲁医院胸外科； 2. 医学院生物化学研究所，山东济南 250012

收稿日期:2005-10-29 修回日期:1900-01-01 发布日期:2006-11-24
通讯作者: 郭旭峰

GUO Xu-feng,HU Guo-qiang,YIN Qiu-wei,ZHAO Jian,CHEN Wei-wen

1. Department of Thoracic Surgery, Qilu Hospital; 2. Institute of Biochemistry, School of Medicine, Shandong University, Jinan 250012, Shandong, China

Received:2005-10-29 Revised:1900-01-01 Published:2006-11-24
Contact: GUO Xu-feng

摘要： 目的：检测非小细胞肺癌（NSCLC）组织中垂体瘤转化基因（PTTG）及血管内皮生长因子C（VEGFC）的表达和微淋巴管密度值（LMVD），探讨它们与NSCLC发生、发展的关系及其临床意义。方法：实时荧光定量PCR和免疫组化分别检测NSCLC中 PTTG、VEGFC mRNA 和蛋白的表达，结合临床病理特征及预后进行分析。同时，对随访资料进行生存分析，绘制生存率曲线，探讨PTTG、VEGFC 对肺癌患者预后的影响。结果：PTTG、VEGFC mRNA 和LMVD值在不同性质肺组织中的表达差别均有统计学意义(P<0.05)，在不同年龄、性别、是否吸烟、肿瘤大小、组织学类型及分化程度组间差别无统计学意义(P>0.05)，在TNM分期、淋巴结转移与否及预后组间差别有统计学意义(P<0.05)。PTTG、VEGFC蛋白表达在淋巴结转移与否及预后组间差别有统计学意义(P<0.05)。生存率曲线显示，PTTG、VEGFC 高表达者生存时间均短于低/无表达者(P=0.030，0.027，n=65)。PTTG表达与VEGFC、LMVD密切相关，随着PTTG表达增加，VEGFC分级及LMVD值亦增加。结论：PTTG、VEGFC 的过表达促使肿瘤微淋巴管生成，进而促进了肿瘤细胞淋巴结转移；PTTG和VEGFC表达可作为判断NSCLC生物学行为及肺癌患者预后的良好指标；VEGFC有望成为肺癌抗淋巴管治疗的新靶点。

关键词: 血管内皮生长因子C, 微淋巴管密度 , 垂体瘤转化基因

Abstract: To investigate the lymphatic microvessel density and expressions of pituitary tumor transforming gene and vascular endothelial growth factor C in nonsmall cell lung cancer(NSCLC)，and explore their correlations to clinicopathologic features of NSCLC． Methods：Realtime fluorescence quantitative PCR and immunohistochemistry method were used to detect the expressions of PTTG, VEGFC and LMVD in NSCLC. We also investigated the effects of PTTG and VEGFC on the prognosis of patients with NSCLC by drawing KaplanMeier curves. Results: There were higher expressions of PTTG,VEGFC and LMVD in NSCLC tissues than in adjacent tissues and benign lesions(P<0.05).　There were no significant differences between the expressions of PTTG,　VEGFC and age, sex, smoking, and size,　histological type and differentiation of tumor(P>0.05), but there were statistical relationships between their expressions and TNM stage, lymph node metastasis and prognosis(P<0.05).　The KaplanMeier curves showed that patients with low or no expressions of PTTG or VEGFC survivedpositively correlated with VEGFC expression and LMVD．　Conclusion: Tumor lymph angiogenesis is enhanced by the overexpression of PTTG and VEGFC; PTTG and VEGFC can be used as two useful markers to judge the biological behavior and the prognosis of lung cancer; VEGFC can be used as a new target of antilymphatic therapy for lung cancer.

Key words: Pituitary tumor transforming gene, Vascular endothelial growth factor C, Lymphatic microvessel density

郭旭峰,陈蔚文,胡国强,尹秋伟,赵健 . PTTG、VEGF-C 基因过表达与非小细胞肺癌发生、发展的关系及其临床意义[J]. 山东大学学报(医学版), 2006, 44(11): 1175-1179.

GUO Xu-feng,HU Guo-qiang,YIN Qiu-wei,ZHAO Jian,CHEN Wei-wen. Overexpressions of PTTG and VEGFC in nonsmall cell lung cancer and their clinical significances[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(11): 1175-1179.

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