脂氧合酶抑制剂NDGA对HT29结肠癌细胞转移影响的研究

山东大学学报(医学版)

脂氧合酶抑制剂NDGA对HT29结肠癌细胞转移影响的研究

夏光涛,张尚忠,张源潮,杨清锐,周立祥

1. 山东大学山东省立医院风湿免疫科，山东济南 250021；2.山东大学齐鲁医院消化科，山东济南 250012；3.宁津县中医院外科，山东宁津 253400

收稿日期:2006-03-29 修回日期:1900-01-01 出版日期:2006-07-24 发布日期:2006-07-24
通讯作者: 夏光涛

XIA Guang-tao,ZHANG Yuan-chao,YANG Qing-rui,ZHANG Shang-zhong,ZHOU Li-xiang

1. Department of Rheumatism, Shandong Provincial Hospital, Shandong University, Jinan 250021,Shandong, China； 2.Department of Gastroenterology, Qilu Hospital of Shandong University, Jinan 250012,Shandong, China

Received:2006-03-29 Revised:1900-01-01 Online:2006-07-24 Published:2006-07-24
Contact: XIA Guang-tao

摘要/Abstract

摘要： 12 3.宁津县中医院外科，山东宁津 253400）目的：探讨脂氧合酶抑制剂去甲二氢愈创木酸（nordihydroguaiaretic acid，NDGA）在体外对结肠癌HT29细胞株转移的影响。方法：应用不同浓度的NDGA处理HT29结肠癌细胞，RTPCR检测血管内皮生长因子（vascular endothelial growth factor，VEGF）,流式细胞仪检测CD44v6表达的变化；扫描电镜观察癌细胞表面微绒毛的变化。结果：经过NDGA作用后，CD44v6、VEGF随着药物浓度的增大，表达逐步下调。扫描电镜检测NDGA处理癌细胞48?h后，癌细胞微绒毛卷曲成小球状。结论：NDGA能够抑制HT29结肠癌细胞转移。

Abstract: To investigate the effect of the lipoxygenase inhibitor, nordihydroguaiaretic acid （NDGA） on metastasis of colon cancer cell line HT29 in vitro. Methods: Different concentrations of NDGA were used in the cancer cell. RTPCR was used to detect vascular endothelial growth factor（VEGF）, flow cytometry to detect the expression of CD44v6, and scanning electron microscope to observe cells microvilli. Results: The expression of CD44v6 went down with the rise of concentration and so was VEGF detected with RTPCR. Through scanning electron microscope, it was found that cells microvilli curled to microball after NDGA had been used for 48 hours. Conclusion: NDGA can suppress the metastasis of colon cancer cellline HT29.

Key words: Lipoxygenase, Colonic neoplasms , Metastasis

夏光涛,张尚忠,张源潮,杨清锐,周立祥. 脂氧合酶抑制剂NDGA对HT29结肠癌细胞转移影响的研究[J]. 山东大学学报(医学版), 2006, 44(7): 714-717.

XIA Guang-tao,ZHANG Yuan-chao,YANG Qing-rui,ZHANG Shang-zhong,ZHOU Li-xiang. [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(7): 714-717.

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