Abstract: To explore the alteration of protein kinase C(PKC) activity and the effect of aminoguanidine (AG) on diabetic glomerulopathy in streptozocin (STZ)induced diabetic rats. Methods： A total of 60 male Wistar rats were randomized into normal control, diabetes control and AG treatment group. Blood glucose, HbA1c，serum creatinine（Cr）, urinary albumin, creatinine clearance rate (Ccr), PKC activity and DAG level of glomerular were measured 8 and 24 weeks after the treatment and compared the results from other groups. Results： The level of blood glucose, HbA1c，urinary albumin, the PKC activity, the Ccr and the DAG level of glomerular were all significantly increased in diabetic rats. AG had no effect on the alteration of blood glucose, HbA1c and weight in diabetic rats, but decreased the level of urinary albumin, the PKC activity, the Ccr and the DAG level of glomerular. Conclusion： The rise of PKC activity plays an important role in the development of diabetic nephropathy. AG could reduce the PKC activity and may be a novel inhibitor of protein kinase C.

Key words: Diabetes mellitus, Aminoguanidine, Protein kinase