SYBR Green实时定量PCR检测人脑原发胶质瘤中LATS1和LATS2基因的表达

SYBR Green实时定量PCR检测人脑原发胶质瘤中LATS1和LATS2基因的表达

Expression of LATS1 and LATS2 in primary glioma by SYBRGreen quantitative realtime PCR

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山东大学学报(医学版)

姜政¹,胡锦²,李新钢³,卢大儒,江玉泉

1．山东大学齐鲁医院神经外科实验室，山东济南 250012；2．上海交通大学第六人民医院神经外科，上海 200233；3．山东大学齐鲁医院神经外科，山东济南 250012；4．复旦大学生命科学院遗传学研究所遗传工程国家重点实验室，上海 200433

收稿日期:2005-10-29 修回日期:1900-01-01 出版日期:2006-12-24 发布日期:2006-12-24
通讯作者: 姜政

JIANG Zheng1， HU Jin2， LI Xingang3，　LU Daru4， JIANG Yuquan3

1. Laboratory of Neurosurgery, Qilu Hospital of Shandong University, Jinan 250012， Shandong, China；2. Department of Neurosurgery, Sixth People′s Hospital, Jiao Tong University, Shanghai 200233, China；

Received:2005-10-29 Revised:1900-01-01 Online:2006-12-24 Published:2006-12-24
Contact: JIANG Zheng

摘要： 目的：运用实时定量PCR检测肿瘤抑制基因LATS1和LATS2的mRNA在人脑原发胶质瘤组织中的表达情况，并进一步分析其表达变化与临床资料之间的关系。方法：建立SYBR Green 实时定量PCR的检测方法；通过标准曲线法对肿瘤抑制基因LATS1和LATS2的mRNA在30例原发胶质瘤（WHO分级：Ⅱ级10例，Ⅲ级10例，Ⅳ级10例）和10例正常脑组织中的表达进行定量检测；统计学分析不同级别胶质瘤及正常脑组织间的表达差异，并进一步结合临床资料分析不同性别和不同年龄组的表达差异。结果：与正常脑组织相比，在各病理级别胶质瘤中LATS1和LATS2的mRNA表达均下调(P＜0.05)，但胶质瘤不同级别组之间的差异均无统计学意义(P＞0.05)。LATS1和LATS2在不同性别组和年龄组胶质瘤中的表达差异也无统计学意义（P＞0.05）。结论：①成功地建立了SYBR Green实时定量PCR检测LATS1和LATS2基因表达的方法；②胶质瘤中LATS1和LATS2的mRNA表达水平均低于正常脑组织，但不同病理分级间的表达无明显差异。

关键词: 实时定量聚合酶链反应, SYBR Green , LATS2, 神经胶质瘤, LATS1

Abstract: To detect the expression of LATS1 and LATS2 mRNA in human primary glioma by SYBR Green quantitative realtime PCR, and to analyse the relationship between the expression and the clinical data. Methods: The method of SYBR Green quantitative realtime PCR was established. The expression of tumor suppressor gene LATS1 and LATS2 in 30 primary glioma tissues (WHO：GradeⅡ10 cases, Grade Ⅲ10 cases and Grade Ⅳ 10 cases) and 10 normal brain tissues were quantitated by the method of standard curve. The expression differences of LATS1 and LATS2 in different grade glioma compared with the normal brain tissues were studied and the relationship of the expression and the clinical data was also analyzed by a statistical method. Results: The expressions of LATS1 and LATS2were all significantly downregulated in different grade glioma compared with normal brain tissues (P＜0.05). But there were no significant differences in the expression of LATS1 and LATS2 among the different grade glioma (P＞0.05). And there were also no significant differences in different gender and age. Conclusions: The method to detect the expression of LATS1 and LATS2 by SYBR Green quantitative realtime PCR is established successfully. The tumor suppressor genes LATS1 and LATS2 are all downregulated in human glioma compared with those in the normal brain tissues.

Key words: Large tumor suppressor, homolog 1, Large tumor suppressor, homolog 2, Glioma, Realtime polymerase chainreaction, SYBR Green

姜政,胡锦,李新钢,卢大儒,江玉泉. SYBR Green实时定量PCR检测人脑原发胶质瘤中LATS1和LATS2基因的表达[J]. 山东大学学报(医学版), 2006, 44(12): 1289-1293.

JIANG Zheng,HU Jin,LI Xingang,LU Daru,JIANG Yuquan. Expression of LATS1 and LATS2 in primary glioma by SYBRGreen quantitative realtime PCR[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2006, 44(12): 1289-1293.

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